Arabidopsis genes involved in acyl lipid metabolism. A 2003 census of the candidates, a study of the distribution of expressed sequence tags in organs, and a Web-based database
Arabidopsis genes involved in acyl lipid metabolism. A 2003 census of the candidates, a study of the distribution of expressed sequence tags in organs, and a Web-based database
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DOI：
10.1104/pp.103.022988
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发表时间：
2003-06-01
期刊：
影响因子：
7.4
通讯作者：
Ohlrogge, JB
中科院分区：
文献类型：
--
作者：
Beisson, F;Koo, AJK;Ohlrogge, JB
拟南芥的基因组中已经搜索了参与酰基脂质代谢的基因序列。超过600个编码蛋白质已被确定，编目，并根据预测的功能，亚细胞位置和选择性剪接分类。这些蛋白质中至少有三分之一以前被注释为“未知功能”或与酰基脂质代谢无关的功能;因此，这项研究改进了200多个基因的注释。特别是，通过对生物化学文献和注释为“脂肪酶”的大量蛋白质的序列的严格检查，已经改进了脂解酶组（总共至少110个成员）的注释。此外，还调查了表达序列标签（EST）数据，并对与这些基因相关的3,700多个EST进行了编目。与特定cDNA文库相关的EST数量的统计分析允许计算不同器官之间差异表达的概率。已经鉴定了超过130个基因，其在种子、叶、根或花中优先表达的统计概率>0.95。所有数据都可以作为基于网络的数据库，拟南芥脂质基因数据库（http：//www.plantbiology.msu.edu/lipids/genesurvey/index.htm）获得。脂质基因目录和EST分析的数据的组合可用于获得对基因家族成员的差异表达和途径特异性基因集的洞察，这反过来将指导研究以了解单个基因的特定功能。
The genome of Arabidopsis has been searched for sequences of genes involved in acyl lipid metabolism. Over 600 encoded proteins have been identified, cataloged, and classified according to predicted function, subcellular location, and alternative splicing. At least one-third of these proteins were previously annotated as "unknown function" or with functions unrelated to acyl lipid metabolism; therefore, this study has improved the annotation of over 200 genes. In particular, annotation of the lipolytic enzyme group (at least 110 members total) has been improved by the critical examination of the biochemical literature and the sequences of the numerous proteins annotated as "lipases." In addition, expressed sequence tag (EST) data have been surveyed, and more than 3,700 ESTs associated with the genes were cataloged. Statistical analysis of the number of ESTs associated with specific cDNA libraries has allowed calculation of probabilities of differential expression between different organs. More than 130 genes have been identified with a statistical probability >0.95 of preferential expression in seed, leaf, root, or flower. All the data are available as a Web-based database, the Arabidopsis Lipid Gene database (http://www.plantbiology.msu.edu/lipids/genesurvey/index.htm). The combination of the data of the Lipid Gene Catalog and the EST analysis can be used to gain insights into differential expression of gene family members and sets of pathway-specific genes, which in turn will guide studies to understand specific functions of individual genes.