TLR-2 Arg753Gln, TLR-4 Asp299Gly, and TLR-4 Thr399Ile polymorphisms in Henoch Schonlein purpura with and without renal involvement
TLR-2 Arg753Gln, TLR-4 Asp299Gly, and TLR-4 Thr399Ile polymorphisms in Henoch Schonlein purpura with and without renal involvement
复制标题
过敏性紫癜伴或不伴肾脏受累的 TLR-2 Arg753Gln、TLR-4 Asp299Gly 和 TLR-4 Thr399Ile 多态性
作者：
A. Soylu;S. Kızıldağ;S. Kavukçu;S. Cingöz;M. Türkmen;B. Demir;M. Sakızlı
感染可引起或加重肾小球肾炎和肾血管炎，如过敏性紫癜（HSP）。HSP在家族性地中海热患者中更常见，其中TLR-2 Arg 753 Gln多态性频率增加。虽然肾脏受累是影响HSP预后的最重要因素，但尚不清楚哪些患者会有肾脏疾病，以及为什么有些患者有严重的肾脏受累，而有些患者有轻度肾脏疾病。我们研究了TLR-2和TLR-4多态性对HSP患者肾脏受累的发生率和严重程度的作用。我们研究了HSP伴和不伴肾炎的患者（每组n = 15）和健康对照组（n = 100）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析TLR-2 Arg 753 Gln和TLR-4 Asp 299 Gly/Thr 399 Ile多态性。TLR-2 Arg 753 Gln、TLR-4 Asp 299 Gly和Thr 399 Ile多态性在健康对照组中的频率分别为1%、3%和2%。与健康对照组相比，这些多态性的频率在HSP伴或不伴肾炎患者中没有差异。TLR-3 Arg 753 Gln、TLR-4 Asp 299 Gly和Thr 399 Ile多态性在HSP或HSP肾炎患者中不增加。
Infections may trigger or aggravate glomerulonephritidis and renal vasculitis like Henoch Schonlein purpura (HSP). HSP is seen more frequently in patients with familial Mediterranean fever in which TLR-2 Arg753Gln polymorphism frequency is increased. Although renal involvement is the most important factor affecting the prognosis in HSP, it is not known which patients will have renal disease or why some patients have severe renal involvement while some others have mild renal disease. We investigated the role of TLR-2 and TLR-4 polymorphisms on the incidence and severity of renal involvement in HSP patients. We studied HSP patients with and without nephritis (n = 15 for each group) and healthy controls (n = 100). TLR-2 Arg753Gln and TLR-4 Asp299Gly/Thr399Ile polymorphisms were analyzed with polymerase chain reaction–restriction fragment length polymorphism method. The frequency of TLR-2 Arg753Gln, TLR-4 Asp299Gly, and Thr399Ile polymorphisms in healthy controls were 1, 3, and 2%, respectively. The frequencies of these polymorphisms were not different in HSP patients with or without nephritis compared to healthy controls. TLR-3 Arg753Gln, TLR-4 Asp299Gly, and Thr399Ile polymorphisms are not increased in HSP or HSP nephritis patients.