New natural product families from an environmental DNA (eDNA) gene cluster
New natural product families from an environmental DNA (eDNA) gene cluster
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DOI：
10.1021/ja0268985
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发表时间：
2002-08-28
影响因子：
15
通讯作者：
Clardy, J
中科院分区：
文献类型：
--
作者：
Brady, SF;Chao, CJ;Clardy, J
未培养的细菌代表了新的和有用的天然产物的潜在丰富来源。研究这些天然产物需要开发有效而直接的方法来获取未培养细菌产生的小分子化学多样性。在这项研究中，直接从土壤样品中提取的DNA（环境DNA，eDNA）被用来构建粘粒文库在土方ichiacoli，然后这些克隆进行了测定的生产pesticsis。一个13开放阅读框（ORF）生物合成基因簇（feeA-M）在一个抗菌活性克隆CSLC-2中发现，赋予E。大肠杆菌生产的两个新的家庭的天然产物，是来自长链N-酰基酪氨酸。本文介绍了费根簇和三个由酪氨酸衍生的长链酰基酚族（族1、2和3）。
Uncultured bacteria represent a potentially rich source of new and useful natural products. Studying these natural products requires the development of effective yet straightforward methods to access the small-molecule chemical diversity produced by uncultured bacteria. In this study, DNA extracted directly from soil samples (environmental DNA, eDNA) was used to construct cosmid libraries inEscherichiacoli, and these clones were then assayed for the production of antibiosis. A 13 open reading frame (ORF) biosynthetic gene cluster (feeA-M) found in one of the antibacterial active clones, CSLC-2, confers toE. colithe production of two new families of natural products that are derived from long chainN-acyltyrosines. Thefeegene cluster and three families of the long chain acyl phenols derived from tyrosine (families1,2, and3) are described.