Generation of a systemic antitumor response with regional intratumoral injections of interferon gamma retroviral vector.
Generation of a systemic antitumor response with regional intratumoral injections of interferon gamma retroviral vector.
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通过局部瘤内注射干扰素γ逆转录病毒载体产生全身抗肿瘤反应。
DOI：
10.1089/hum.1998.9.15-2231
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发表时间：
1998
影响因子：
4.2
通讯作者：
T. Fong
中科院分区：
文献类型：
--
作者：
L. Karavodin;J. Robbins;K. Chong;D. Hsu;C. Ibañez;S. Mento;D. Jolly;T. Fong
The generation of a lasting systemic immune response is a primary goal for cancer immunotherapy. Here we examine the ability of high-titer IFN-gamma retroviral vector injected into an accessible tumor to generate significant antitumor responses at a distal untreated site. CT26 or B16F10 murine tumors were inoculated subcutaneously to form solid tumors in BALB/c or C57BL/6 mice. Seven to 10 days postinoculation, high-titer IFN-gamma retroviral vector was directly injected into the subcutaneous tumor nodule, and optimal dose and course of therapy were determined. As a model for disseminated disease, mice were inoculated intravenously with CT26 cells to form pulmonary lesions, at the same time as the subcutaneous injections. Regression of subcutaneous tumor correlated with a systemic response at the distal lung metastases in the IFN-gamma-treated group (p < 0.0005). Splenocytes from mice with completely regressed tumors had a twofold increase in percent specific cytotoxicity in a standard CTL assay as compared with nonresponding mice. CD8+ T cells were shown to be essential for the regional and systemic antitumor response, as determined by in vivo cell depletion experiments. These data demonstrate that IFN-gamma retroviral vector gene therapy delivered intralesionally can generate significant inhibition of pulmonary tumor formation distal to the treatment site. The data from these preclinical studies suggest the potential clinical value of retroviral vector-mediated cytokine gene therapy for systemic cancer.
腺病毒介导的头颈鳞状细胞癌基因治疗。
DOI：
10.1177/000348949610500713
发表时间：
1996
期刊：
The Annals of otology, rhinology, and laryngology
影响因子：
--
作者：
Goebel,EA;Davidson,BL;Zabner,J;Graham,SM;Kern,JA
通讯作者：
Kern,JA
用两种插入 IFN-γ 基因的肿瘤细胞对荷瘤小鼠进行抗转移疫苗接种。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Porgador,A;Bannerji,R;Watanabe,Y;Feldman,M;Gilboa,E;Eisenbach,L
通讯作者：
Eisenbach,L
用过继转移的 T 细胞治疗皮下肿瘤。
DOI：
10.1006/cimm.1997.1124
发表时间：
1997
期刊：
Cellular immunology.
影响因子：
--
作者：
Peng,L;Shu,S;Krauss,JC
通讯作者：
Krauss,JC