β-catenin defines head versus tail identity during planarian regeneration and Homeostasis
β-catenin defines head versus tail identity during planarian regeneration and Homeostasis
复制标题
DOI：
10.1126/science.1150029
复制
发表时间：
2008-01-18
期刊：
影响因子：
56.9
通讯作者：
Alvarado, Alejandro Sanchez
中科院分区：
文献类型：
--
作者：
Gurley, Kyle A.;Rink, Jochen C.;Alvarado, Alejandro Sanchez
截肢后，淡水浮游生物会从造成的前部或后部伤口适当地再生头部或尾巴。区分前部和后部的机制以及指导适当的缺失身体部分的替换的机制尚不清楚。我们发现，在甲虫中，β-连环素或蓬乱的RNA干扰(RNAi)会导致后部截肢时头部而不是尾巴的不适当再生。相反，β-连环蛋白拮抗剂腺瘤性息肉病结肠的RNAi导致前部伤口的尾巴再生。此外，β-连环素的沉默足以将未切割的成年动物的尾巴转变为头部。我们认为，β-连环蛋白作为一个分子开关，在浮游生物的再生和动态平衡过程中，指定和维持前后的特性。
After amputation, freshwater planarians properly regenerate a head or tail from the resulting anterior or posterior wound. The mechanisms that differentiate anterior from posterior and direct the replacement of the appropriate missing body parts are unknown. We found that in the planarian Schmidtea mediterranea, RNA interference ( RNAi) of beta-catenin or dishevelled causes the inappropriate regeneration of a head instead of a tail at posterior amputations. Conversely, RNAi of the beta-catenin antagonist adenomatous polyposis coli results in the regeneration of a tail at anterior wounds. In addition, the silencing of beta-catenin is sufficient to transform the tail of uncut adult animals into a head. We suggest that beta-catenin functions as a molecular switch to specify and maintain anteroposterior identity during regeneration and homeostasis in planarians.