Lateral Spacing of TiO2 Nanotubes Modulates Osteoblast Behavior
Lateral Spacing of TiO2 Nanotubes Modulates Osteoblast Behavior
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DOI：
10.3390/ma12182956
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发表时间：
2019-09-01
期刊：
影响因子：
3.4
通讯作者：
Cimpean, Anisoara
中科院分区：
文献类型：
--
作者：
Necula, Madalina Georgiana;Mazare, Anca;Cimpean, Anisoara
二氧化钛（TiO 2）纳米管涂层基质具有上级骨整合性能和局部药物释放能力，在许多方面彻底改变了植入物的概念。虽然越来越多的报道描述了纳米管直径对细胞行为的影响，但关于纳米管横向间距对参与骨再生的细胞的影响知之甚少。在这种情况下，在本研究中的MC 3 T3-E1小鼠前成骨细胞的行为进行了研究，通过使用TiO 2纳米管的直径类似于78 nm和横向间距分别为18 nm和80 nm。两种纳米结构表面都以大致相等的程度支持细胞活力和增殖。然而，在细胞铺展面积，形态，肌动蛋白细胞骨架的组织和粘着斑的模式，注意到明显的差异。此外，前成骨细胞分化潜能的研究表明，较大间距的纳米结构增强碱性磷酸酶、骨桥蛋白和骨钙素成骨细胞特异性标志物诱导成骨分化的表达的能力更高。这些发现提供了证据，证明在设计具有改善的生物学性能的骨植入物时，必须考虑TiO 2纳米管涂层钛（Ti）表面的横向间距。
Titanium dioxide (TiO2) nanotube coated substrates have revolutionized the concept of implant in a number of ways, being endowed with superior osseointegration properties and local drug delivery capacity. While accumulating reports describe the influence of nanotube diameter on cell behavior, little is known about the effects of nanotube lateral spacing on cells involved in bone regeneration. In this context, in the present study the MC3T3-E1 murine pre-osteoblast cells behavior has been investigated by using TiO2 nanotubes of similar to 78 nm diameter and lateral spacing of 18 nm and 80 nm, respectively. Both nanostructured surfaces supported cell viability and proliferation in approximately equal extent. However, obvious differences in the cell spreading areas, morphologies, the organization of the actin cytoskeleton and the pattern of the focal adhesions were noticed. Furthermore, investigation of the pre-osteoblast differentiation potential indicated a higher capacity of larger spacing nanostructure to enhance the expression of the alkaline phosphatase, osteopontin and osteocalcin osteoblast specific markers inducing osteogenic differentiation. These findings provide the proof that lateral spacing of the TiO2 nanotube coated titanium (Ti) surfaces has to be considered in designing bone implants with improved biological performance.