ALK7 is a novel marker for adipocyte differentiation
ALK7 is a novel marker for adipocyte differentiation
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DOI：
10.2152/jmi.53.238
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发表时间：
2006-08-01
影响因子：
0.7
通讯作者：
Sugino, Hiromu
中科院分区：
文献类型：
--
作者：
Kogame, Masahiro;Matsuo, Shinji;Sugino, Hiromu
转化生长因子-β (TGF-β) 家族成员调节多种细胞功能并在细胞分化中发挥重要作用。激活素受体样激酶 7 (ALK7) 是 TGF-β 家族成员的受体，最初作为孤儿受体从大鼠中克隆，最近被证明是 nodal、激活素 B 和激活素 AB 的 I 型受体。 ALK7不仅在神经元中表达，还在产生胰岛素的胰岛β细胞以及白色和棕色脂肪组织中表达；然而，ALK7 在这些组织中的具体功能尚不清楚。为了测试ALK7是否参与脂肪细胞分化，我们分析了其在脂肪细胞分化过程中的表达。通过逆转录聚合酶链式反应（RT-PCR）、Western blotting和免疫荧光染色在3T3-L1细胞中检测脂肪细胞分化后期的ALK7表达。我们还通过 RT-PCR 检测了基质血管成分 (SVF) 细胞中 ALK7 的表达。这些结果表明 ALK7 是一种在脂肪细胞分化后期特异性表达的新标记物。此外，我们的结果表明节点或激活素 B 可能参与脂肪细胞分化。
Transforming growth factor-beta (TGF-beta) family members regulate a variety of cellular functions and play important roles in cell differentiation. Activin receptor-like kinase 7 (ALK7), a receptor for TGF-beta family members, was initially cloned from rats as an orphan receptor and has been recently shown to be a type I receptor for nodal, activin B and activin AB. ALK7 is expressed not only in neurons, but also in insulin-producing islet beta cells and white and brown adipose tissues; however, the specific functions of ALK7 in these tissues are not known. In order to test whether ALK7 is involved in adipocyte differentiation, we analyzed its expression during adipocyte differentiation. ALK7 expression was detected in the late phase of adipocyte differentiation by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence staining in 3T3-L1 cells. We also detected the expression of ALK7 by RT-PCR in stromal vascular fraction (SVF) cells. These results indicated that ALK7 is a novel marker specifically expressed during the late phase of adipocyte differentiation. Furthermore, our results suggest the possible involvement of nodal or activin B in adipocyte differentiation.