N-acetyl-D-glucosamine induces germination in Candida albicans through a mechanism sensitive to inhibitors of cAMP-dependent protein kinase
N-acetyl-D-glucosamine induces germination in Candida albicans through a mechanism sensitive to inhibitors of cAMP-dependent protein kinase
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DOI：
10.1016/s0898-6568(98)00015-1
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发表时间：
1998-11-01
影响因子：
4.8
通讯作者：
Cantore, ML
中科院分区：
文献类型：
--
作者：
Castilla, R;Passeron, S;Cantore, ML
本研究通过评估两种通透性蛋白激酶(PKA)抑制剂对N-乙酰-D-氨基葡萄糖(GlcNAc)和血清诱导的白念珠菌分化的影响，探讨了cAMP依赖的蛋白激酶(PKA)在白念珠菌二型转变中的作用。在体外抑制白色念珠菌PKA的热稳定性PKA抑制剂(MyrPKI)的透性肉豆蔻酰化衍生物对GlcNAc诱导的培养物萌发有浓度依赖性的抑制作用；无论加入该抑制剂的时间长短，萌发都停止。MyrPKI也能抑制二丁酰cAMP(DBcAMP)和胰升糖素刺激的萌发，但不影响血清诱导的萌发，另一种高度特异的PKA抑制剂H-89对萌发也有同样的作用。MyrPKI和H-89对酵母细胞的萌发均无影响。综上所述，我们的结果表明，cAMP介导的PKA激活在形态发生的生化机制中起着关键作用。手机信号10；10：713-719,1998。(C)1998年爱思唯尔科学公司。
The present study examines the involvement of cAMP-dependent protein kinase (PKA) in the dimorphic transition of Candida albicans by assessing the in vivo effect of two permeable PKA inhibitors on N-acetyl-D-glucosamine (GlcNAc)- and serum-induced differentiation. The permeable myristoylated derivative of the heat-stable PKA inhibitor (MyrPKI), which inhibited C. albicans PKA in vitro, caused a concentration-dependent inhibition of germ-tube formation in cultures induced to germinate by GlcNAc; germination halted irrespective of the time of addition of the inhibitor. MyrPKI also blocked dibutyryl-cAMP (dbcAMP)- and glucagon-stimulated germination but did not affect serum-induced germination H-89, another highly specific PKA inhibitor, displayed the same effect on germination. Neither MyrPKI nor H-89 had any effect on budding of yeast cells. In conclusion, our results indicate that cAMP-mediated activation of PKA plays a pivotal role in the biochemical mechanism underlying morphogenesis. CELL SIGNAL 10;10:713-719, 1998. (C) 1998 Elsevier Science Inc.