Expression profile of ADAM10 and ADAM17 in allergic rhinitis
Expression profile of ADAM10 and ADAM17 in allergic rhinitis
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DOI：
10.1002/alr.21614
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发表时间：
2015-11-01
影响因子：
6.4
通讯作者：
Bae, Woo Yong
中科院分区：
文献类型：
--
作者：
Chueh, Hee Won;Park, Seong Kook;Bae, Woo Yong
背景资料：去整合素和金属蛋白酶（ADAM）是多功能基因家族，其有助于细胞外基质的稳态、特异性细胞内信号的转导、器官形成、炎症、组织重塑、粘附和细胞迁移。ADAM 17是最具特征的脱落酶，具有广泛的推定底物，包括各种炎症调节剂。在蛋白质序列和催化结构域的结构特性方面，ADAM 10是与ADAM 17最相似的ADAM。本研究的目的是探讨ADAM 10和ADAM 17在变应性鼻炎中的表达，以了解其各自的作用。方法：观察变应性鼻炎和非变应性鼻炎鼻粘膜中ADAM 10和ADAM 17的表达。组织样本通过逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法进行评价，并采用光密度法对数据进行半定量分析。ADAM 17信使RNA（mRNA）水平显著高于对照组（P <0.05）。（p < 0.001）变应性鼻粘膜低于非变应性鼻粘膜，而非过敏性鼻粘膜中的ADAM 10 mRNA水平显著降低（p < 0.001）。结论：变应性鼻炎患者鼻黏膜中ADAM 17蛋白表达降低，而非变应性鼻炎患者鼻黏膜中ADAM 10蛋白表达升高，可能通过未知的途径参与变应性鼻炎的发生发展。我们认为，了解ADAM 17和ADAM 10的表达谱可能有助于阐明变应性鼻炎的发病机制。(C)2015 ARS-AAOA，LLC.
Background: A disintegrin and metalloprotease (ADAM) is a multifunctional gene family that contributes to the homeostasis of the extracellular matrix, transduction of specific intracellular signals, organogenesis, inflammation, tissue remodeling, adhesion, and cell migration. ADAM17 is the best characterized sheddase, with widespread putative substrates, including various inflammatory modulators. ADAM10 is the most similar ADAM to ADAM17 in terms of both protein sequence and the structural properties of their catalytic domains. The objective of this work was to assess the expression of ADAM10 and ADAM17 in allergic rhinitis to gain insight into their respective roles.Methods: The expression of ADAM10 and ADAM17 was investigated in the nasal mucosa under allergic and nonallergic conditions. Tissue samples were evaluated by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting, and data were analyzed semiquantitatively with densitometry.Results: The ADAM17 messenger RNA (mRNA) level was significantly (p < 0.001) lower in the allergic nasal mucosa than in the nonallergic nasal mucosa, whereas the ADAM10 mRNA level was significantly (p < 0.001) lower in the nonallergic nasal mucosa. The ADAM17 protein levels were also significantly (p < 0.001) lower in the allergic nasal mucosa, whereas the ADAM10 protein levels were lower in the nonallergic nasal mucosa (p = 0.002).Conclusion: Decreased expression of ADAM17 and increased expression of ADAM10 may contribute to the development of allergic rhinitis through unknown pathways. We suggest that understanding the expression profile of ADAM17 and ADAM10 might help to elucidate the mechanism of allergic rhinitis. (C) 2015 ARS-AAOA, LLC.