Detection of pathogenic gram negative bacteria using infrared thermography
Detection of pathogenic gram negative bacteria using infrared thermography
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DOI：
10.1016/j.infrared.2012.07.011
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发表时间：
2012-11-01
影响因子：
3.3
通讯作者：
Philip, John
中科院分区：
文献类型：
--
作者：
Lahiri, B. B.;Divya, M. P.;Philip, John
活细菌的检测在微生物学和生物技术的所有领域中是最重要的。传统的细菌计数方法通常耗时且劳动密集。所有生物体由于代谢活动而产生热量，因此，热能的测量是用于检测和定量细菌的可行工具。本文采用一种非接触的、真实的时间测量方法--红外热像法（IRT），对四种临床上重要的革兰氏阴性致病菌，即霍乱弧菌、拟态弧菌、奇异变形杆菌和铜绿假单胞菌的温度变化进行了测量。我们观察到，能量含量，定义为细菌代谢活动产生的热量与从液体介质损失到周围的热量的比率，在所有四种病原菌中随细菌浓度线性变化。在不同物种中观察到的能量含量的量归因于它们的代谢和形态，这些代谢和形态影响对流速度，从而影响介质中的热传输。(C)2012爱思唯尔有限公司版权所有。
Detection of viable bacteria is of prime importance in all fields of microbiology and biotechnology. Conventional methods of enumerating bacteria are often time consuming and labor-intensive. All living organisms generate heat due to metabolic activities and hence, measurement of heat energy is a viable tool for detection and quantification of bacteria. In this article, we employ a non-contact and real time method - infrared thermography (IRT) for measurement of temperature variations in four clinically significant gram negative pathogenic bacteria, viz. Vibrio cholerae, Vibrio mimicus, Proteus mirabilis and Pseudomonas aeruginosa. We observe that, the energy content, defined as the ratio of heat generated by bacterial metabolic activities to the heat lost from the liquid medium to the surrounding, vary linearly with the bacterial concentration in all the four pathogenic bacteria. The amount of energy content observed in different species is attributed to their metabolisms and morphologies that affect the convection velocity and hence heat transport in the medium. (C) 2012 Elsevier B.V. All rights reserved.