Differential expression of semaphorin 3A and its receptors during mouse retinal development
Differential expression of semaphorin 3A and its receptors during mouse retinal development
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DOI：
10.1002/cbf.2833
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发表时间：
2012-10
影响因子：
3.6
通讯作者：
Ji-Ae Ko;Y. Mizuno;M. Shibasaki;K. Yamane;T. Chikama;K. Sonoda;Y. Kiuchi
中科院分区：
文献类型：
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作者：
Ji-Ae Ko;Y. Mizuno;M. Shibasaki;K. Yamane;T. Chikama;K. Sonoda;Y. Kiuchi
脑信号蛋白不仅在发育过程中的轴突导向中起作用，而且还有助于各种其他生物过程。我们现在已经研究了脑信号蛋白3A（Sema 3A）及其受体成分神经纤毛蛋白1（Npn 1）和丛蛋白A（PlxA）在小鼠视网膜发育过程中的表达。免疫组织荧光分析显示，Sema 3A和Npn 1的表达模式在胚胎和出生后的发展是相似的。PlxA的表达模式也类似于Sema 3A和Npn 1在胚胎和出生后早期（睁眼前）的发展。然而，PlxA的表达模式发生了显着变化后，眼睛打开，与表达消失，从视神经和增加的强度在视网膜色素上皮细胞。免疫沉淀分析表明，Sema 3A相互作用与PlxA在视网膜色素上皮细胞系ARPE 19，但不是在视网膜神经节细胞系RGC 5，而相反的关联模式是明显的Sema 3A和Npn 1。考虑到大气中的氧气被认为在各种眼部细胞类型的分化和维持中发挥作用，我们的研究结果表明，通过环境氧气对PlxA表达的影响激活的Sema 3A-PlxA信号传导可能有助于视网膜色素上皮的分化。版权所有© 2012约翰威利父子有限公司.
Semaphorins not only function in axon guidance during development but also contribute to various other biological processes. We have now examined the expression of semaphorin 3A (Sema3A) and its receptor components neuropilin 1 (Npn1) and plexin A (PlxA) during development of the mouse retina. Immunohistofluorescence analysis revealed that the expression patterns of Sema3A and Npn1 were similar during embryonic and postnatal development. The expression pattern of PlxA was also similar to those of Sema3A and Npn1 during embryonic and early postnatal (before eye opening) developments. However, the pattern of PlxA expression changed markedly after eye opening, with the expression disappearing from the optic nerve and increasing in intensity in the retinal pigment epithelium. Immunoprecipitation analysis showed that Sema3A interacted with PlxA in the retinal pigment epithelial cell line ARPE19 but not in the retinal ganglion cell line RGC5, whereas the opposite pattern of association was apparent for Sema3A and Npn1. Given that atmospheric oxygen is thought to play a role in the differentiation and maintenance of various ocular cell types, our results suggest that Sema3A–PlxA signalling activated by an effect of ambient oxygen on PlxA expression may contribute to differentiation of the retinal pigment epithelium. Copyright © 2012 John Wiley & Sons, Ltd.