Habituation of glomerular responses in the olfactory bulb following prolonged odor stimulation reflects reduced peripheral input.
Habituation of glomerular responses in the olfactory bulb following prolonged odor stimulation reflects reduced peripheral input.
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DOI：
10.3389/fnmol.2015.00053
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发表时间：
2015
影响因子：
4.8
通讯作者：
Fletcher ML
中科院分区：
文献类型：
--
作者：
Ogg MC;Bendahamane M;Fletcher ML
长时间的气味刺激后，嗅球（OB）二尖瓣/簇状（M/T）细胞的输出会因随后的嗅觉刺激而减少。目前，尚不清楚这种减少是否是外周嗅觉感觉神经元（OSN）反应适应的功能，还是反映了灯泡回路的抑制。我们在麻醉的转基因 GCaMP2 小鼠中使用宽视野钙成像来比较 30 秒气味刺激前后的兴奋性肾小球层气味反应。使用我们的方案检测到随后的肾小球气味反应对相同和结构相似的气味剂的显着习惯化。为了测试 OSN 末端的抑制是否会导致这种习惯化，在 OSN 周围气味没有激活的情况下，使用嗅觉神经层 (ON) 刺激来驱动肾小球层反应。气味习惯化后，与气味诱发的肾小球反应相反，ON刺激诱发的肾小球反应并未习惯。气味习惯后气味和电刺激之间的反应差异提供了证据，表明在 OB 肾小球层中测量到的气味反应减少很可能是外周发生的 OSN 适应过程的结果。
Following prolonged odor stimulation, output from olfactory bulb (OB) mitral/tufted (M/T) cells is decreased in response to subsequent olfactory stimulation. Currently, it is unclear if this decrease is a function of adaptation of peripheral olfactory sensory neuron (OSN) responses or reflects depression of bulb circuits. We used wide-field calcium imaging in anesthetized transgenic GCaMP2 mice to compare excitatory glomerular layer odor responses before and after a 30-s odor stimulation. Significant habituation of subsequent glomerular odor responses to both the same and structurally similar odorants was detected with our protocol. To test whether depression of OSN terminals contributed to this habituation, olfactory nerve layer (ON) stimulation was used to drive glomerular layer responses in the absence of peripheral odor activation of the OSNs. Following odor habituation, in contrast to odor-evoked glomerular responses, ON stimulation-evoked glomerular responses were not habituated. The difference in response between odor and electrical stimulation following odor habituation provides evidence that odor response reductions measured in the glomerular layer of the OB are most likely the result of OSN adaptation processes taking place in the periphery.