Immunohistochemical demonstration of proliferating lymphatic vessels in colorectal carcinoma and its clinicopathological significance
Immunohistochemical demonstration of proliferating lymphatic vessels in colorectal carcinoma and its clinicopathological significance
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DOI：
10.1016/j.canlet.2006.02.013
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发表时间：
2007-02-08
期刊：
影响因子：
9.7
通讯作者：
Nakayama, Jun
中科院分区：
文献类型：
--
作者：
Omachi, Toshiya;Kawai, Yoshiko;Nakayama, Jun
通过淋巴管向区域淋巴结的淋巴转移是多种恶性肿瘤预后不良的重要指标。最近，淋巴管生成因其在各种癌症的肿瘤进展中可能发挥的作用而受到广泛关注。然而，尚未报道结直肠癌中淋巴管活跃增殖的形态学证据。在这里，我们首先设计了一种三重免疫染色方法来检测增殖的淋巴管，利用Ki-67抗原抗体作为细胞增殖标记物，细胞角蛋白抗体作为上皮细胞标记物，以及podoplanin抗体作为淋巴管特异性标记物。 Ki-67/podoplanin 免疫反应性使我们能够识别增殖的淋巴管，而细胞角蛋白免疫反应性使我们能够将增殖的淋巴管与淋巴管腔中的 Ki-67/细胞角蛋白阳性癌细胞区分开。使用该技术分析 64 名结直肠癌患者的样本，我们发现结直肠癌组织中的淋巴管密度和淋巴管增殖活性均较正常组织显着增加。然后我们检查了淋巴管生成程度与患者预后或临床病理学变量之间的相关性，但在这些分析中没有获得统计学上的显着差异。因此，这些结果结合在一起表明结直肠癌中发生广泛的淋巴管生成，但单独的淋巴管生成程度并不是该疾病的独立预后因素。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
Lymphatic metastasis to the regional lymph nodes through the lymphatic vessels is an important indicator of poor prognosis in many types of malignant tumors. Recently, much attention has been paid to lymphangiogenesis for its possible role on tumor progression in various carcinomas. However, morphological evidence that lymphatic vessels actively proliferate in colorectal carcinoma has not been reported. Here, we first devised a triple immunostaining method to detect proliferating lymphatic vessels utilizing antibody to Ki-67 antigen as a marker of cell proliferation, antibody to cytokeratin as an epithelial cell marker, and antibody to podoplanin as a lymphatic vessel-specific marker. Ki-67/podoplanin-immunoreactivity enabled us to identify proliferating lymphatic vessels, while cytokeratin immunoreactivity allowed us to distinguish proliferating lymphatic vessels from Ki-67/cytokeratin-positive carcinoma cells in lymphatic lumens. Analyzing 64 colorectal carcinoma patients' samples using this technique, we showed that both lymphatic vessel density and proliferating activity of lymphatic vessels were significantly increased in colorectal carcinoma tissues compared with their normal counterparts. We then examined the correlation between the degree of lymphangiogenesis and patients' prognosis or clinicopathological variables, but no statistically significant differences were obtained in these analyses. Thus, these results combined together indicate that extensive lymphangiogenesis occurs in colorectal carcinoma, but that the degree of lymphangiogenesis alone is not an independent prognostic factor for this disease. (c) 2006 Elsevier Ireland Ltd. All rights reserved.