A Replisome's journey through the bacterial chromosome.
A Replisome's journey through the bacterial chromosome.
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DOI：
10.3389/fmicb.2015.00562
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发表时间：
2015
影响因子：
5.2
通讯作者：
Reyes-Lamothe R
中科院分区：
文献类型：
--
作者：
Beattie TR;Reyes-Lamothe R
基因组复制需要一个多组分机器（复制体）的协调活动。与整个细菌结构域的代谢多样性背景相反，细菌复制体的组成和结构似乎在进化过程中几乎没有发生变化。这种不变性强调了复制体复制基因组的效率。它还强调了复制体固有的各种策略的成功，以应对压力和避免在DNA合成的关键阶段出现问题。在这里，我们总结了目前的细菌复制体结构的理解，并突出不同的细菌类群的已知变化。然后，我们研究了确保细菌复制体在DNA上正确组装的机制，在延伸过程中保持在一起，并在终止时分解。我们提出的想法，复制体的架构可能更灵活，以前认为和推测的复制体的元素，保持其稳定性，以确保从起点到终点的安全旅程。
Genome duplication requires the coordinated activity of a multi-component machine, the replisome. In contrast to the background of metabolic diversity across the bacterial domain, the composition and architecture of the bacterial replisome seem to have suffered few changes during evolution. This immutability underlines the replisome’s efficiency in copying the genome. It also highlights the success of various strategies inherent to the replisome for responding to stress and avoiding problems during critical stages of DNA synthesis. Here we summarize current understanding of bacterial replisome architecture and highlight the known variations in different bacterial taxa. We then look at the mechanisms in place to ensure that the bacterial replisome is assembled appropriately on DNA, kept together during elongation, and disassembled upon termination. We put forward the idea that the architecture of the replisome may be more flexible that previously thought and speculate on elements of the replisome that maintain its stability to ensure a safe journey from origin to terminus.