EFFECT OF EXTRACELLULAR CA2+ AND CA2+-ANTAGONISTS ON THE MOVEMENT AND CHEMOORIENTATION OF MALE GAMETES OF ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)
EFFECT OF EXTRACELLULAR CA2+ AND CA2+-ANTAGONISTS ON THE MOVEMENT AND CHEMOORIENTATION OF MALE GAMETES OF ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)
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DOI：
10.1111/j.1438-8677.1994.tb00820.x
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发表时间：
1994-12-01
期刊：
影响因子：
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通讯作者：
CALENBERG, M
中科院分区：
文献类型：
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作者：
MAIER, I;CALENBERG, M
硅水仙雄性配子响应性信息素的化学定向受到两种不同机制的影响，即趋化作用和趋化性。这些特征是前向鞭毛和瞬态的强烈不对称弯曲模式；后鞭毛分别单侧弯曲。细胞外 Ca2+ 的去除表明正常的鞭毛运动和趋化作用需要培养基中具有毫摩尔浓度的 Ca2+。 La3+ 强烈抑制对信息素的反应，而 Ca2+ 通道药物维拉帕米和硝苯地平则几乎没有作用。尽管如此，硝苯地平仍然有效地抑制了信息素源的积累。这些结果被解释为两种药理学不同的 Ca2+ 通道参与趋化作用和趋化性的指示。钙调蛋白拮抗剂三氟拉嗪在低浓度下会诱导与信息素相同的趋化反应鞭毛反应，其作用机制尚不清楚。
Chemoorientation in male gametes of Ectocarpus siliculosus in response to sexual pheromones is effected by two distinct mechanisms, chemokinesis and chemoklinotaxis. These are characterized by a strongly asymmetric bending pattern of the anteriorly-directed flagellum and transient; unilateral bending of the hind flagellum, respectively. Removal of extracellular Ca2+ showed that normal flagellar movement and chemokinesis require millimolar concentrations of Ca2+ in the medium. The response to pheromones is strongly inhibited by La3+, whereas the Ca2+-channel drugs, verapamil and nifedipine, have only little effect. Nifedipine nethertheless effectively inhibited accumulation at pheromone sources. These results are interpreted as an indication for the involvement of two pharmacologically distinct Ca2+-channels in chemokinesis and chemoklinotaxis. The calmodulin-antagonist, trifluoperazine, induces, at low concentrations, the same flagellar response in chemokinesis as the pheromone, the mechanism of action remaining unknown.