Detecting action potentials in neuronal populations with calcium imaging
Detecting action potentials in neuronal populations with calcium imaging
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DOI：
10.1006/meth.1999.0774
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发表时间：
1999-06-01
期刊：
影响因子：
--
通讯作者：
Yuste, R
中科院分区：
文献类型：
--
作者：
Smetters, D;Majewska, A;Yuste, R
研究神经回路需要同时记录神经元群体活动的方法。在这里，使用新皮质脑片的钙成像，我们利用神经元中普遍存在的钙通道的分布来开发一种方法来重建发生在神经元群体中的动作电位。结合钙成像和神经元的全细胞或穿孔斑片记录，我们证明了每个动作电位在锥体神经元的体细胞中产生一个刻板的钙瞬变。这些信号在没有平均的情况下是可以检测到的，并且信噪比足以重建数百个神经元的尖峰模式，直到相对较高的放电频率。原则上，这项技术可以系统地应用于在体外和体内跟踪神经元群体的活动。(C)1999年学术出版社。
The study of neural circuits requires methods for simultaneously recording the activity of populations of neurons. Here, using calcium imaging of neocortical brain slices we take advantage of the ubiquitous distribution of calcium channels in neurons to develop a method to reconstruct the action potentials occurring in a population of neurons. Combining calcium imaging with whole-cell or perforated patch recordings from neurons loaded with acetoxymethyl ester or potassium salt forms of calcium indicators, we demonstrate that each action potential produces a stereotyped calcium transient in the somata of pyramidal neurons. These signals are detectable without averaging, and the signal-to-noise is sufficient to carry out a reconstruction of the spiking pattern of hundreds of neurons, up to relatively high firing frequencies. This technique could in principle be applied systematically to follow the activity of neuronal populations in vitro and in vivo. (C) 1999 Academic Press.