ZmDWF1 regulates leaf angle in maize
ZmDWF1 regulates leaf angle in maize
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ZmDWF1 调节玉米叶角
DOI：
10.1016/j.plantsci.2022.111459
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发表时间：
2022-09-18
期刊：
影响因子：
5.2
通讯作者：
Ku, Lixia
中科院分区：
文献类型：
--
作者：
Cao, Yingying;Dou, Dandan;Ku, Lixia
叶角是玉米高密度栽培条件下提高籽粒产量的重要农艺性状。近年来，人们开展了大量的研究来研究导致LA变异的数量性状基因座/基因，但通过基于图谱的克隆只发现了少数基因。在这里，我们克隆了ZmDWF1基因，该基因编码油菜素类固醇(BRS)生物合成途径中的&UDelta；24-甾醇还原酶。ZmDWF1的过表达导致LA增大，表明ZmDWF1是玉米LA的正调控基因。为了揭示ZmDWF1的调控框架，我们进行了RNA测序和酵母双杂交(Y2H)筛选分析。RNA测序分析结果表明，ZmDWF1主要影响与细胞壁相关代谢和激素代谢相关的基因的表达水平，包括BR、赤霉素和生长素。经BiFc筛选证实有3种蛋白(ZmPP2C-1、ZmROF1和ZmTWD1)与ZmDWF1相互作用。我们揭示了ZmDWF1基因控制玉米植株构型的一个新的调控网络。
Leaf angle (LA) is a critical agronomic trait enhancing grain yield under high-density planting in maize. A number of researches have been conducted in recent years to investigate the quantitative trait loci/genes responsible for LA variation, while only a few genes were identified through map-based cloning. Here we cloned the ZmDWF1 gene, which was previously reported to encode & UDelta;24-sterol reductase in the brassinosteroids (BRs) biosynthesis pathway. Overexpression of ZmDWF1 resulted in enlarged LA, indicating that ZmDWF1 is a positive regulator of LA in maize. To reveal the regulatory framework of ZmDWF1, we conducted RNA-Sequencing and yeast-two hybrid (Y2H) screening analysis. RNA-Sequencing analyzing results indicate ZmDWF1 mainly affected expression level of genes involved in cell wall associated metabolism and hormone metabolism including BR, gibberellin, and auxin. Y2H screening with Bi-FC assay confirmed three proteins (ZmPP2C-1, ZmROF1, and ZmTWD1) interacting with ZmDWF1. We revealed a new regulatory network of ZmDWF1 gene in controlling plant architecture in maize.