Nanoprobes with near-infrared persistent luminescence for in vivo imaging
Nanoprobes with near-infrared persistent luminescence for in vivo imaging
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DOI：
10.1073/pnas.0702427104
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发表时间：
2007-05-29
影响因子：
11.1
通讯作者：
Scherman, Daniel
中科院分区：
文献类型：
--
作者：
de Chermont, Quentin le Masne;Chaneac, Corinne;Scherman, Daniel
荧光越来越多地用于体内成像，并提供了显着的结果。然而，该技术提出了一些局限性，尤其是由于在外部照明下组织自动荧光和低波长激发光的组织穿透弱。我们通过使用适合小动物成像的持续发光纳米颗粒开发了一种替代的光学成像技术。这些纳米颗粒可以在注射前激发，并且可以实时遵循其体内分布超过1小时，而无需任何外部照明源。纳米颗粒表面的化学修饰导致肺或肝脏靶向或持久的血液循环。肿瘤质量也可以在小鼠模型上鉴定。
Fluorescence is increasingly used for in vivo imaging and has provided remarkable results. Yet this technique presents several limitations, especially due to tissue autofluorescence under external illumination and weak tissue penetration of low wavelength excitation light. We have developed an alternative optical imaging technique by using persistent luminescent nanoparticles suitable for small animal imaging. These nanoparticles can be excited before injection, and their in vivo distribution can be followed in real-time for more than 1 h without the need for any external illumination source. Chemical modification of the nanoparticles' surface led to lung or liver targeting or to long-lasting blood circulation. Tumor mass could also be identified on a mouse model.