Quantitative on-line concentration for capillary electrophoresis with inkjet sample introduction technique
Quantitative on-line concentration for capillary electrophoresis with inkjet sample introduction technique
复制标题
喷墨进样技术毛细管电泳在线定量浓缩
DOI：
10.1002/jssc.201500201
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Katsumi Uchiyama
中科院分区：
文献类型：
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作者：
Ying Rang;Hulie Zeng;Hizuru Nakajima;Shungo Kato;Katsumi Uchiyama
将一种基于喷墨进样的定量进样方法应用于毛细管电泳中，并结合在线富集技术和在线扫描富集技术。甲基黄嘌呤用作该方法概念验证的模型化合物。通过控制进样过程中喷射液滴的数量，可以很容易地控制进样量。在优化条件下，当进样液滴数小于100时，进样液滴数与峰面积呈线性关系。在优化的定量在线浓缩条件下，可可碱、咖啡因和茶碱的检测限分别为1.0、2.0和1.0 μ M。通过与传统注射方法的比较，对喷墨注射系统进行了评价。喷墨进样模式的电泳图与流体动力学进样模式的电泳图相同，与电动进样模式相比没有观察到样品区分。建立的方法用于瓶装绿色茶中甲基黄嘌呤的测定。可可碱、咖啡因和茶碱的回收率分别为94.1%、110.6%和86.8%。我们的结论是，所提出的方法可以用于定量浓缩毛细管电泳，从而导致在提高准确度。
A quantitative sample introduction method based upon inkjet injection was applied to capillary electrophoresis coupled with stacking and sweeping on‐line concentration techniques. Methylxanthines were used as model compounds for the proof‐of‐concept of the method. The volume of injected sample could be easily manipulated by controlling the number of ejected droplets in the injection procedure. Under optimized conditions, a linear relationship between the ejected droplet number and peak area was obtained when the droplet number introduced into the capillary was less than 100. Under optimized quantitative on‐line concentration conditions, the limits of detection for theobromine, caffeine, and theophylline were 1.0, 2.0, and 1.0 μM, respectively. The inkjet injection system was evaluated by comparing it with conventional injection methods. The electropherogram of the inkjet injection mode was the same as that for hydrodynamic injection mode, and no sample discrimination was observed compared with the electrokinetic injection mode. The established method was applied to the determination of methylxanthines in bottled green tea. The recoveries of theobromine, caffeine, and theophylline were 94.1, 110.6, and 86.8%, respectively. We conclude that proposed method can be used for quantitative concentration for capillary electrophoresis, thus resulting in an improved accuracy.