Effects of benzyladenine and α-Naphthaleneacetic acid on the formation of protocorm-like bodies (PLBs) from explants of outer tissue of Cymbidium PLBs cultured in vitro
Effects of benzyladenine and α-Naphthaleneacetic acid on the formation of protocorm-like bodies (PLBs) from explants of outer tissue of Cymbidium PLBs cultured in vitro
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DOI：
10.2503/jjshs.68.35
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发表时间：
1999-01-01
影响因子：
--
通讯作者：
Kako, S
中科院分区：
文献类型：
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作者：
Fujii, K;Kawano, M;Kako, S
从大花蕙兰类原球茎（Protocorm-like bodies，PLBs）上切取外层组织（Outer tissue，OT）外植体，在不加任何激素的MS培养基上，或在含有BA和NAA的MS培养基上，诱导出类原球茎。0.5或5.0 μ M NAA和2.5或5.0 μ M BA的组合，在Gym的OT外植体中，有77.5%的外植体产生原球茎。感恩节的“本土”在健身房。用2.5 μ M的BA处理幸运彩虹‘Lapine Dancer’，73.7%的OT外植体产生原球茎，在BA或BA + NAA培养基上比在无激素培养基上快。单独添加NAA可显著抑制原球茎的形成。在MS + BA或BA + NAA培养基上，OT诱导的原球茎形成比在无激素培养基上快，在附加NAA或BA的培养基上，原球茎大小与原球茎产量呈极显著负相关（r=-0.893，r=-0.843）。BA促进了小型原球茎的形成，而NAA抑制了原球茎的形成。但是，原球茎一旦形成，NAA就有增大原球茎的趋势。
Explants of outer tissue (OT) excised from protocorm-like bodies (PLBs) of Cymbidium produced PLBs on Murashige and Skoog (MS) medium without any hormone, or those containing benzyladenine (BA) and alpha-naphthaleneacetic acid (NAA). The combination of 0.5 or 5.0 mu M NAA and 2.5 or 5.0 mu M BA yielded PLBs by 77.5% OT explants in Gym. Thanksgiving 'Nativity'. In Gym. Lucky Rainbow 'Lapine Dancer' a treatment with 2.5 mu M BA yielded PLBs from 73.7% OT explants and faster on a medium with BA or BA + NAA than on a hormone-free medium. An addition of NAA alone inhibited PLB formation significantly. Thus, PLB formed from OT faster on MS media supplemented with BA or a combination of BA and NAA than on a hormone-free medium.In media supplemented with NAA or BA, there was a high negative correlation between PLB size and yield from an outer tissue (r=-0.893, r=-0.843). BA promoted formation of small sized PLBs, whereas NAA suppressed PLB formation. However, once the PLBs were formed, NAA tended to increase their sizes.