Allosamidin, a chitinase inhibitor produced by Streptomyces, acts as an inducer of chitinase production in its producing strain
Allosamidin, a chitinase inhibitor produced by Streptomyces, acts as an inducer of chitinase production in its producing strain
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DOI：
10.2183/pjab.77.79
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发表时间：
2001-05-01
影响因子：
3.1
通讯作者：
Sakuda, S
中科院分区：
文献类型：
--
作者：
Nakanishi, E;Okamoto, S;Sakuda, S
阿洛酰胺是由链霉菌产生的几丁质酶抑制剂。产生别沙菌素的链霉菌经常在土壤中发现。阿洛酰胺菌素是一种亲水性化合物，但仅存在于其产生菌的菌丝体中，外源阿洛酰胺菌素被吸附在其产生菌的菌丝体上。我们发现，外源性添加的allosamidin的培养基与胶体几丁质作为唯一的碳源可以强烈地加速几丁质酶的生产由allosamidin生产链霉菌在低浓度，如1 μ M。在另一方面，allosamidin抑制几丁质酶活性的培养链霉菌lividans，allosamidin-nonproducing微生物。这些结果表明，allosamidin在其生产者中具有生理作用，作为几丁质酶生产的诱导剂，这可能有助于生产者在富含几丁质的环境如土壤中利用几丁质。
Allosamidin is a chitinase inhibitor produced by Streptomyces. Allosamidin-producing Streptomyces is frequently found in soil. Allosamidin is a hydrophilic compound, but is found only in mycelia of its producer, and exogenous allosamidin is adsorbed on mycelia of its producing strain. We found that exogenous addition of allosamidin to a culture medium with colloidal chitin as a sole carbon source can strongly accelerate the chitinase production by an allosamidin-producing Streptomyces at low concentrations such as 1 muM. On the other hand, allosamidin depressed the chitinase activity in the culture of Streptomyces lividans, an allosamidin-nonproducing microbe. These results suggest that allosamidin has a physiological role in its producer as an inducer of chitinase production, which may be useful for chitin utilization by the producer in chitin-rich environment such as in soil.