Moc3, a novel Zn finger type protein involved in sexual development, ascus formation, and stress response of Schizosaccharomyces pombe
Moc3, a novel Zn finger type protein involved in sexual development, ascus formation, and stress response of Schizosaccharomyces pombe
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DOI：
10.1007/s00294-005-0028-z
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发表时间：
2005-12-01
期刊：
影响因子：
2.5
通讯作者：
Kawamukai, M
中科院分区：
文献类型：
--
作者：
Goldar, MM;Jeong, HT;Kawamukai, M
在粟酒裂殖酵母中，cAMP途径是启动有性发育的主要营养感受途径。我们鉴定了moc 1-moc 4作为克服部分不育S.粟酒裂殖酵母菌株由于cAMP的升高。在比较同一品种诱导性发育能力的强弱时，对粟酒裂殖酵母菌株Moc 1的影响最大，Moc 2的影响最小，Moc 3和Moc 4的影响居中。已知Moc 1/Sds 23和Moc 2/Ded 1分别是M期进展的潜在调节剂和必需的RNA解旋酶。Moc 3（SPAC 821.07c）是一种新的含有Zn（2）-Cys（6）基序的锌指蛋白。构建了moc 3基因的缺失突变体，发现其突变体的交配效率较低，并形成了异常的子囊。此外，出乎意料的是，moc 3破坏剂对CaCl 2和DNA损伤剂如MMS和UV敏感。这些表型与zfs 1干扰物中观察到的表型相反，与moc 1干扰物中观察到的表型完全不同。Moc 3定位于细胞核中，如对Zfs 1所观察到的。Moc 3与Moc 4/Zfs 1在双杂交系统中弱结合，但在同一分析中没有其他Moc（s）组合相互结合。因此，Moc 3不仅参与性发育，而且以独立于Moc 1和Moc 2的方式参与生殖细胞的形成和DNA完整性。粟酒
The cAMP pathway in Schizosaccharomyces pombe is the major nutrient sensing pathway to initiate sexual development when opposite mating type cells exist. We identified moc1-moc4 as genes that overcome a partially sterile S. pombe strain due to an elevation of cAMP. When we compared the strength of inducing ability of sexual development in the same S. pombe strain, Moc1 had highest, Moc2 had lowest, and both Moc3 and Moc4 had intermediate effects. Moc1/Sds23 and Moc2/Ded1 are known to be a potential regulator of M-phase progression and an essential RNA helicase, respectively. While Moc4 was found to be identical with a Zn-finger protein Zfs1, Moc3 (SPAC821.07c) was a novel protein containing a Zn-finger (Zn(2)-Cys(6)) motif. Deletion mutant of the moc3 gene was constructed and its disruptant was found to be lower in mating efficiency and formed aberrant asci. In addition, unexpectedly, a moc3 disruptant was sensitive to CaCl2 and DNA damaging agents such as MMS and UV. Those phenotypes were opposite to the phenotypes observed in a zfs1 disruptant, and quite different from the ones in a moc1 disruptant. Moc3 localized in the nucleus as observed for Zfs1. Moc3 bound with Moc4/Zfs1 weakly in the two hybrid system, but no other combination of Moc(s) bound each other in the same analysis. Thus, Moc3 is not only involved in sexual development, but also in ascus formation and DNA integrity in an independent manner with Moc1 and Moc2 in S. pombe.