Reduced oxidative stress increases acute cold stress tolerance in zebrafish
Reduced oxidative stress increases acute cold stress tolerance in zebrafish
复制标题
DOI：
10.1016/j.cbpa.2019.06.009
复制
发表时间：
2019-09-01
影响因子：
2.3
通讯作者：
Du, Zhen-Yu
中科院分区：
文献类型：
--
作者：
Lu, Dong-Liang;Ma, Qiang;Du, Zhen-Yu
在自然界和水产养殖业中，冷应激都是对鱼类的一大威胁，并可诱导多种鱼类的氧化应激。而氧化应激在寒冷导致的死亡中的确切作用仍不清楚。本研究的目的是评价氧化应激对鱼类耐冷性的影响，并验证氧化状态的改变是否会影响鱼类的耐冷性。我们首次证明了急性冷暴露诱导了斑马鱼肝脏的高氧化应激，这可能导致死亡。然后，我们分别进行了体内和体外实验，以确定改变的氧化状态对斑马鱼和斑马鱼肝细胞系(ZFL)耐寒性的影响。在体内研究中，饲喂α-硫辛酸或还原型谷胱甘肽的斑马鱼具有较低的冷氧化应激和组织损伤，表现出较高的耐寒性。在斑马鱼细胞实验中，过氧化氢增加氧化应激降低了细胞的耐冷性，而添加维生素C(VC)降低氧化应激可部分恢复细胞的耐冷性。综上所述，我们得出的结论是，氧化应激的减少提高了鱼类的耐寒性。
Cold stress is a major threat to fish in both nature and aquaculture, and can induce oxidative stress in various fish. While the exact role of oxidative stress in cold-caused mortality is still unknown. The purpose of the present study was to evaluate the effects of oxidative stress on cold tolerance in fish and verify whether changing oxidative status could affect cold tolerance. We firstly demonstrated that acute cold exposure induced high oxidative stress in zebrafish liver, which may lead to mortality. Then we performed in vivo and in vitro experiments to determine the effects of the altered oxidative status on cold tolerance in zebrafish and zebrafish liver cell line (ZFL), respectively. In the in vivo study, the zebrafish which were fed with alpha-lipoic acid or reduced glutathione had lower cold-caused oxidative stress and tissues damage, and showed higher cold tolerance. In the experiment using zebrafish cells, increasing oxidative stress by H2O2 decreased the cellular cold tolerance, and the cold tolerance was partly recovered when oxidative stress was reduced by the addition of Vitamin C (VC). Taken together, we conclude that the reduction of oxidative stress increases cold tolerance in fish.