Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults: Isomers of C8 Health Project in China
Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults: Isomers of C8 Health Project in China
复制标题
成人肝功能生物标志物紊乱与全氟烷基酸暴露相关：中国C8健康项目异构体
DOI：
10.1016/j.envres.2019.02.013
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发表时间：
2019-05-01
影响因子：
8.3
通讯作者：
Dong, Guang-Hui
中科院分区：
文献类型：
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作者：
Nian, Min;Li, Qing-Qing;Dong, Guang-Hui
暴露于化学物质可能会影响肝酶，增加肝脏疾病的风险。全氟烷基酸（PFAA）是一种持久性有机污染物，对生物体具有肝毒性作用。然而，数据是稀缺的，以表征特定结构的PFAA异构体在一般人群中的肝毒性作用。为了解决这一数据缺口，我们在中国C8异构体健康项目中评估了血清PFAAs浓度与肝功能生物标志物之间的相关性。采用高效液相色谱-串联质谱法（HPLC-MS/MS）测定了沈阳市1605名成年居民血清中除直链和支链PFOA/PFOS异构体外的18种PFAAs，以及9种全氟羧酸（PFCAs）和2种全氟磺酸（PFSAs）。采用全自动血液生化分析仪测定血清中9种肝功能指标。使用线性回归评估PFAA和连续肝功能生物标志物之间的相关性，并使用逻辑回归评估每个临床参考区间的二分标志物。结果表明，血清PFAAs浓度与肝脏生物标志物水平相关，提示肝毒性，特别是肝细胞损伤。例如，全氟辛酸（PFOA）暴露量增加1 ln单位与血清丙氨酸氨基转移酶（ALT）水平升高7.4% [95%置信区间（CI）：3.9%，11.0%]相关。有趣的是，我们观察到支链PFAA异构体和肝脏生物标志物之间的关联。例如，单位内支链全氟辛烷磺酸异构体暴露量增加一次，ALT水平增加4.3%（95% CI：1.2%，7.4%），ALT异常几率增加33.0%（95% CI：5.0%，67.0%）。此外，我们还发现PFNA与ALT [（6.2%，95% CI：3.1%，9.4%）和AST水平（2.5%，95% CI：0.5%，4.5%）]呈正相关。Logistic回归分析结果显示，PFPeA、PFHxA、PFNA、PFDoDA、PFTrDA和PFTeDA与前白蛋白异常有统计学意义。总之，我们的研究结果支持以前的研究表明PFAA暴露和肝功能生物标志物之间的关联。我们发现了新的证据表明支链全氟辛酸异构体暴露与临床相关的肝细胞功能障碍的风险有关。
Exposure to chemicals may affect liver enzyme to increase the risk of liver diseases. Perfluoroalkyl acids (PFAAs) are one kind of persistent organic pollutants with hepatotoxic effect in organism. However, data is scarce to characterize the hepatotoxic effects of specific structural PFAA isomers in general population. To address this data gap, we evaluated the association between serum PFAAs concentration and liver function biomarkers in the Isomers of C8 Health Project in China. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to measure 18 serum PFAAs, except for linear and branched isomers of PFOA/PFOS, nine perfluorinated carboxylic acids (PFCAs) and two perfluorinated sulfonic acids (PFSAs) were also included, in 1605 adult residents of Shenyang, China. Values for nine serum liver function biomarkers were determined by full-automatic blood biochemical analyzer. Linear regression was used to evaluate associations between PFAAs and continuous liver function biomarkers and logistic regression to assess markers dichotomized per clinical reference intervals. Results indicated that serum PFAAs concentrations were associated with liver biomarker levels suggestive of hepatotoxicity, especially for liver cell injury. For example, a 1 ln-unit increase in total-perfluorooctanoic acid (PFOA) exposure was associated with a 7.4% [95% confidence interval (CI): 3.9%, 11.0%] higher alanine aminotransferase (ALT) level in serum. Interestingly, we observed association between branched PFAA isomers and liver biomarkers. For example, one In-unit increase in branched perfluorooctane sulfonate (PFOS) isomers exposure was associated with a 4.3% increase in ALT level (95% CI: 1.2%, 7.4%) and a 33.0% increased odds of having abnormal ALT (95% CI: 5.0%, 67.0%). Also, we found that PFNA had positive association with ALT [(6.2%, 95% CI: 3.1%, 9.4%) and AST levels (2.5%, 95% CI: 0.5%, 4.5%)]. Logistic regression results showed that PFPeA, PFHxA, PFNA, PFDoDA, PFTrDA and PFTeDA had statistically association with abnormal prealbumin. Conclusively, our results support previous studies showing association between PFAAs exposure and liver function biomarkers. We found new evidence that branched PFAAs isomer exposure is associated with the risk of clinically relevant hepatocellular dysfunction.