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DOI：
10.4049/jimmunol.134.5.2989
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发表时间：
1985-05
影响因子：
4.4
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中科院分区：
文献类型：
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两种与人T、B和NK细胞反应的单克隆抗体（HB-10和HB-11）可识别成人血液中约50%的Leu-3+ T辅助（TH）细胞。在本研究中，HB-11+和HB-11-TH细胞亚群的功能能力进行了检查后，通过荧光激活细胞分选纯化。两个亚群增殖反应PHA，Con A，PWM，和OKT-3抗体。HB-11+ TH细胞对可溶性破伤风类毒素抗原的增殖反应最小，而HB-11-TH细胞反应良好。在有丝分裂原活化后，HB-11+和HB-11-TH细胞均产生诱导大B细胞增殖的可溶性因子。然而，PWM刺激的HB-11+TH细胞不能诱导B细胞分化为分泌抗体的浆细胞，而HB-11-TH细胞在这方面是有效的。结果表明，HB-11抗原表达在原始TH细胞的亚群上，所述原始TH细胞可以产生B细胞生长因子，但缺乏产生B细胞分化因子的能力。
Two monoclonal antibodies (HB-10 and HB-11), which react with human T, B, and NK cells, identify approximately 50% of the Leu-3+ T helper (TH) cells in adult blood. In the present studies, the functional capabilities of the HB-11+ and HB-11-TH cell subpopulations were examined after purification by fluorescence-activated cell sorting. Both subpopulations proliferated in response to PHA, Con A, PWM, and OKT-3 antibodies. The HB-11+ TH cells gave a minimal proliferative response to soluble tetanus toxoid antigen, whereas HB-11-TH cells responded well. After mitogen activation, both HB-11+ and HB-11-TH cells and to produce soluble factors which induce large B cells to proliferate. However, PWM-stimulated HB-11+TH cells were incapable of inducing B cells to differentiate into antibody-secreting plasma cells, whereas HB-11-TH cells were efficient in this regard. The results suggest that the HB-11 antigen is expressed on a subpopulation of virgin TH cells that can produce B cell growth factors but are deficient in the ability to produce B cell differentiation factors.