Immunocytochemical evidence that endometrial stromal granulocytes are granulated lymphocytes
Immunocytochemical evidence that endometrial stromal granulocytes are granulated lymphocytes
复制标题
免疫细胞化学证据表明子宫内膜基质粒细胞是粒状淋巴细胞
作者：
J. Bulmer;Debra Hollings;A. Ritson
子宫内膜间质粒细胞（EGs）在黄体晚期子宫内膜和早孕蜕膜中非常重要。它们被认为是由子宫内膜间质细胞发育而来，并分泌松弛素。最近的免疫组织化学研究表明，EG来源于骨髓，但这一直难以证明，主要是因为特征性的细胞质颗粒不能保存在冷冻组织。两个独立的方法，现在已经采用调查的细胞谱系的EG。通过免疫过氧化物酶法，用4种与常规固定和处理的组织反应的单克隆抗体（mAb）标记孕早期蜕膜的福尔马林固定石蜡包埋切片。此外，用一组mAb标记蜕膜细胞悬液的丙酮固定涂片。将切片和涂片复染以显示EG的特征性细胞质颗粒。子宫内膜粒细胞为LCA+、CD 2+、MT 1+和UCHL 1+，这提供了它们是白细胞的证据。EG可能是大颗粒淋巴细胞系列的成员，并可能在正常着床和胎盘形成中起重要作用。
Endometrial stromal granulocytes (EGs) are prominent in late luteal phase human endometrium and in early pregnancy decidua. They have been believed to develop from endometrial stromal cells and to secrete relaxin. Recent immunohistochemical studies have suggested that EGs are derived from bone marrow but this has been difficult to prove, mainly because the characteristic cytoplasmic granules are not preserved in frozen tissues. Two separate approaches have now been employed to investigate the cellular lineage of EGs. Formalin‐fixed paraffin‐embedded sections of first trimester decidua were labelled by an immunoperoxidase method with four monoclonal antibodies (mAbs) reactive with routinely fixed and processed tissues. In addition, acetone‐fixed smears of decidual cell suspensions were labelled with a panel of mAbs. Sections and smears were counterstained to demonstrate the charateristic cytoplasmic granules of EGs. Endometrial granulocytes were LCA+, CD2+, MT1+, and UCHL1+, which provides evidence that they are leucocytes. EGs are probably members of the large granular lymphocyte series and may have an essential role in normal implantation and placentation.
DOI：
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发表时间：
1985
期刊：
The American journal of pathology
影响因子：
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作者：
Kabawat,SE;Mostoufi-Zadeh,M;Driscoll,SG;Bhan,AK
通讯作者：
Bhan,AK