Sperm from neonatal mammalian testes grafted in mice
Sperm from neonatal mammalian testes grafted in mice
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DOI：
10.1038/nature00918
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发表时间：
2002-08-15
期刊：
影响因子：
64.8
通讯作者：
Schlatt, S
中科院分区：
文献类型：
--
作者：
Honaramooz, A;Snedaker, A;Schlatt, S
精子发生是一个多产和高度有组织的过程，在成年期产生几乎无限数量的精子。生殖细胞的持续增殖和分化发生在与其他睾丸隔室的微妙平衡中，特别是支持的支持细胞(1)。由于缺乏合适的体外或体内模型，人类和大型动物睾丸功能的许多复杂方面仍然难以捉摸。生殖细胞移植在啮齿类动物中产生了完全的供体来源精子（2-6），但在其他哺乳动物中没有（7-9）。在未成熟哺乳动物睾丸的移植组织中以及跨物种的精子生产尚未完成。本文报道了通过将新生小鼠、猪或山羊的睾丸组织移植到小鼠宿主体内建立完整精子发生的方法。这种方法保持了结构的完整性，并为研究和操纵睾丸功能以及保存男性生殖系提供了必要的可达性。结果表明，该方法适用于多种哺乳动物物种。
Spermatogenesis is a productive and highly organized process that generates virtually unlimited numbers of sperm during adulthood. Continuous proliferation and differentiation of germ cells occur in a delicate balance with other testicular compartments, especially the supporting Sertoli cells(1). Many complex aspects of testis function in humans and large animals have remained elusive because of a lack of suitable in vitro or in vivo models. Germ cell transplantation has produced complete donor-derived spermatogenesis in rodents(2-6) but not in other mammalian species(7-9). Production of sperm in grafted tissue from immature mammalian testes and across species has not yet been accomplished. Here we report the establishment of complete spermatogenesis by grafting testis tissue from newborn mice, pigs or goats into mouse hosts. This approach maintains structural integrity and provides the accessibility that is essential for studying and manipulating the function of testes and for preserving the male germ line. Our results indicate that this approach is applicable to diverse mammalian species.