SYNEMIN - A NEW HIGH MOLECULAR-WEIGHT PROTEIN ASSOCIATED WITH DESMIN AND VIMENTIN FILAMENTS IN MUSCLE
SYNEMIN - A NEW HIGH MOLECULAR-WEIGHT PROTEIN ASSOCIATED WITH DESMIN AND VIMENTIN FILAMENTS IN MUSCLE
复制标题
DOI：
10.1016/0092-8674(80)90549-8
复制
发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
LAZARIDES, E
中科院分区：
文献类型：
--
作者：
GRANGER, BL;LAZARIDES, E
一个23万道尔顿多肽通过解聚和聚合与中间纤维亚基，聚蛋白和波形蛋白，从鸟类平滑肌共纯化。这种蛋白质存在于骨骼肌中，与肌球蛋白和丝蛋白不同。利用免疫自显像特异的抗血清对培养细胞进行双免疫荧光显微镜观察，发现该蛋白与desmin和vimentin具有相同的空间分布。在骨骼肌形成过程中，所有3种抗原最初以波浪形细丝的形式存在于细胞质中的多核肌管中。在成肌细胞融合后的一周内，它们开始在肌原纤维Z椎间盘的周围融合，达到在成熟肌肉中观察到的分布，在Z平面内形成相互连接的环状网络。用colcolide处理培养的肌管会导致这3种蛋白的丝状形式共同聚集成细胞质束，但当它们与Z盘相关时，对它们几乎没有影响。用非离子型洗涤剂和高盐提取细胞后，细胞骨架中含有去精蛋白、波形蛋白和23万道尔顿多肽，具有彼此难以区分的免疫荧光模式。这种高分子量蛋白显然与肌细胞中的聚乳酸蛋白和波形蛋白丝密切相关。这种蛋白质被命名为synemin，来源于希腊语中的丝。
A 230,000 dalton polypeptide co-purifies through cycles of depolymerization and polymerization with intermediate filament subunits, desmin and vimentin, from avian smooth muscle. This protein is present in skeletal muscle and is distinct from myosin and filamin. Double immunofluorescence microscopy of cultured cells, using antisera shown to be specific by immunoautoradiography, revealed that this protein has the same spatial distribution as desmin and vimentin. During skeletal myogenesis, all 3 antigens exist initially in multinucleate myotubes as wavy filaments throughout the cytoplasm. Within a week after myoblast fusion, they begin to coalesce at the peripheries of the myofibril Z discs, attaining the distribution observed in mature muscle, a network of interlinked rings within the Z plane. Treatment of cultured myotubes with colcemide causes the filamentous forms of these 3 proteins to co-aggregate into cytoplasmic bundles, but has little effect on them when they are associated with the Z discs. Extraction of cells with nonionic detergent and high salt leaves cytoskeletons containing desmin, vimentin and the 230,000 dalton polypeptide with immunofluorescent patterns that are indistinguishable from one another. This high MW protein apparently is closely associated with desmin and vimentin filaments in muscle cells. The protein was named synemin, from the Greek meaning with filament.