Changes induced by sucrose administration on glucose metabolism in pancreatic islets in normal hamsters
Changes induced by sucrose administration on glucose metabolism in pancreatic islets in normal hamsters
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DOI：
10.1677/joe.0.1710551
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发表时间：
2001-12-01
影响因子：
4
通讯作者：
Gagliardino, JJ
中科院分区：
文献类型：
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作者：
Massa, ML;Borelli, MI;Gagliardino, JJ
我们将葡萄糖诱导的胰岛素分泌的变化与正常蔗糖喂养仓鼠胰岛中葡萄糖代谢和己糖激酶/葡萄糖激酶活性的变化相关联。在正常雄性仓鼠中测量血糖和胰岛素水平，所述正常雄性仓鼠在饮用水中喂食（S5）或不喂食（C5）10%蔗糖5周。分离的胰岛（胶原酶消化）分别用于研究胰岛素分泌、D-[U-C-14]-葡萄糖和D-[5-H-3]-葡萄糖的（CO2）-C-14和（H2O）-H-3产生，培养基中葡萄糖浓度为3.3或16.7 nM，和己糖激酶/葡萄糖激酶活性尽管S5和C5动物具有相当的正常血糖水平，S5仓鼠的胰岛素水平高于C5仓鼠（2.3 +/- 0.1 vs 0.6 +/- 0.03 ng/ml，P
We correlated the changes in glucose-induced insulin secretion with those observed in glucose metabolism and hexokinase/glucokinase activity in islets from normal sucrose-fed hamsters. Blood glucose and insulin levels were measured in normal male hamsters fed with (S5) or without (C5) 10% sucrose in the drinking water for 5 weeks. Isolated islets (collagenase digestion) from both groups of animals were used to stud), insulin secretion, (CO2)-C-14 and (H2O)-H-3 production from D-[U-C-14]-glucose and D-[5-H-3]-glucose respectively, with 3.3 or 16.7 nM glucose in the medium, and hexokinase/glucokinase activity (fluorometric assay) in islet homogenates.Whereas S5 and C5 animals had comparable normal blood glucose levels, S5 showed higher insulin levels than C5 hamsters (2.3 +/- 0.1 vs 0.6 +/- 0.03 ng/ml, P