A mutant of sindbis virus with a host-dependent defect in maturation associated with hyperglycosylation of E2.
A mutant of sindbis virus with a host-dependent defect in maturation associated with hyperglycosylation of E2.
复制标题
辛德毕斯病毒的一种突变体，具有与 E2 的高糖基化相关的宿主依赖性成熟缺陷。
DOI：
10.1016/0042-6822(84)90190-9
复制
发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Stollar,V
中科院分区：
文献类型：
--
作者：
Durbin,RK;Stollar,V
辛德比斯病毒 (SV) 在白纹伊蚊细胞上连续传代后，分离出突变体 (SVap15,21)，该突变体在鸡细胞中产生小噬斑并且对温度敏感 (ts)。在 34.5° 时，这种突变体在蚊子细胞中复制正常，但在小鸡或 BHK 细胞中复制得很差。在脊椎动物细胞中，SVap15/21 在 34.5° 和 40° 处都是 RNA+，并且根据在 40° 处进行的互补测试，被分配到互补组 E。该突变体的复制受阻，与互补组 E 的原型突变体 ofts20 一样，处于核衣壳包膜水平。发现 SVap15/21 的 PE2 和 E2 糖蛋白相对于亲本病毒 (SVstd) 的相应糖蛋白高度糖基化。对 SVap15/21 回复体的分析表明 PE2 和 E2 高糖基化与病毒成熟过程中宿主特异性缺陷之间存在因果关系。病毒粒子组装中的宿主特异性缺陷与病毒结构蛋白的高糖基化的关联表明宿主特异性糖基化模式在确定病毒宿主范围中的潜在重要性。
Following serial passage of Sindbis virus (SV) onAedes albopictusmosquito cells a mutant (SVap15,21) was isolated which in chick cells produced small plaques and was temperature sensitive (ts). At 34.5° this mutant replicated normally in mosquito cells, but only poorly in chick or BHK cells. In the vertebrate cells SVap15/21was RNA+at both 34.5 and 40° and on the basis of complementation tests carried out at 40°, was assigned to complementation group E. The block in the replication of this mutant, like that ofts20 the prototype mutant of complementation group E, was at the level of nucleocapsid envelopment. The PE2 and E2 glycoproteins of SVap15/21were found to be hyperglycosylated relative to the corresponding glycoproteins of the parent virus (SVstd). Analysis of revertants of SVap15/21suggests a causal relationship between PE2 and E2 hyperglycosylation and the host-specific defect in virus maturation. The association of a host-specific defect in virion assembly with hyperglycosylation of a viral structural protein points to the potential importance of host-specific glycosylation patterns in the determination of viral host range.