Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion
Self-assembling peptide and nHA/CTS composite scaffolds promote bone regeneration through increasing seed cell adhesion
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自组装肽和 nHA/CTS 复合支架通过增加种子细胞粘附促进骨再生
DOI：
10.1016/j.msec.2018.07.079
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lixin Zhu
中科院分区：
文献类型：
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作者：
Zhiming Zhang;Guofeng Wu;Yanlin Cao;Chun Liu;Yanglei Jin;Yihan Wang;Lianjun Yang;Jiasong Guo;Lixin Zhu
以纳米羟基磷灰石（nHA）和壳聚糖（CTS）为原料制备的多孔支架在骨组织工程中得到了广泛的应用。然而，nHA/CTS支架中的细胞粘附相对较差，这也不能为种子细胞提供理想的三维环境。这些缺陷限制了这些BTE支架修复骨缺损的适用性。为了解决这些挑战，我们设计了一种复合支架，将nHA/CTS与自组装肽（SAP）结合在一起，SAP是一种类似于细胞外基质的材料。我们发现SAP/nHA/CTS支架既增加了骨髓间充质干细胞（BMSCs）的粘附，又增强了支架的力学性能。然后将该复合支架用于修复小鼠模型中的股骨髁骨缺损。12周后，使用H&E染色、微计算机断层扫描和骨矿物质密度测试证明愈合和矿化。据我们所知，这是首次报道SAP/nHA/CTS支架可以增加细胞粘附，促进股骨髁骨缺损的重建。此外，这项研究表明，BTE使用SAP/nHA/CTS支架可能是一种新的前瞻性策略，用于愈合广泛的骨缺损。
Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE). However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells. These deficiencies limit the applicability of these BTE scaffolds to repair bone defects. To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix. We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold. This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model. Healing and mineralization was demonstrated after 12 weeks using H&E staining, microcomputerized tomography, and bone mineral density tests. To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects. Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.