Combination of FSH priming and hCG priming for in-vitro maturation of human oocytes
Combination of FSH priming and hCG priming for in-vitro maturation of human oocytes
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DOI：
10.1093/humrep/deg335
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发表时间：
2003-08-01
影响因子：
6.1
通讯作者：
Chen, PH
中科院分区：
文献类型：
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作者：
Lin, YH;Hwang, JL;Chen, PH
背景技术背景：本研究的目的是确定在体外成熟（IVM）计划中，除了hCG预激外，FSH预激是否有任何额外的益处。方法：60例多囊卵巢综合征（PCOS）患者接受了68个IVM周期，根据计算机生成的数字随机分配接受FSH刺激或不接受FSH刺激。35个周期用75 IU rFSH预处理6天，33个周期未预处理。每个周期取卵前36 h给予hCG 10 000 IU。未成熟的卵母细胞在体外成熟并通过ICSI受精，并在第2天或第3天更换所得胚胎。结果：共回收未成熟卵母细胞1528个。总成熟率和受精率分别为74.2%和72.8%。胚胎移植后，23例妊娠（33.8%）。FSH预处理组和非FSH预处理组的卵母细胞数量和子宫内膜厚度相似。FSH预处理组注射hCG当天的血清雌二醇水平显著高于非FSH预处理组（377.2 pmol/l vs 143.8 pmol/l，P = 0.001）。FSH预处理组的成熟率、受精率和妊娠率分别为76.5%、75.8%和31.4%，而非FSH预处理组的成熟率、受精率和妊娠率分别为71.9%、69.5%和36.4%（均不显著）。结论：IVM是治疗PCOS的一种可行的方法。FSH预激对IVM没有额外的有益作用。
BACKGROUND: The purpose of this study was to determine if there is any additional benefit from FSH priming in addition to hCG priming on in-vitro maturation (IVM) programmes. METHODS: Sixty women with polycystic ovary syndrome (PCOS) who underwent 68 IVM cycles were randomized by computer-generated numbers to receive FSH stimulation or not. Thirty-five cycles were pretreated with 75 IU rFSH for 6 days, and 33 cycles were not. Every cycle was given hCG 10 000 IU 36 h before oocyte retrieval. Immature oocytes were matured in vitro and fertilized by ICSI, and the resulting embryos were replaced on day 2 or 3. RESULTS: A total of 1528 immature oocytes were recovered. The overall maturation and fertilization rates were 74.2 and 72.8% respectively. After embryo transfer, 23 pregnancies resulted (33.8%). The oocyte numbers and endometrial thickness were similar between FSH-primed and non-FSH-primed groups. Serum estradiol level on the day of hCG injection was significantly higher in the FSH-primed group than in the non-FSH-primed group (377.2 pmol/l versus 143.8 pmol/l, P = 0.001). The maturation rate, fertilization rate and pregnancy rate were 76.5, 75.8 and 31.4% respectively for FSH-primed group, and 71.9, 69.5 and 36.4% respectively for non-FSH-primed group (all not significant). CONCLUSIONS: IVM is a feasible treatment for women with PCOS. FSH priming has no additional beneficial effect on IVM.