In-situ grafting hydrophilic polymer on chitosan modified poly (dimethylsiloxane) microchip for separation of biomolecules
In-situ grafting hydrophilic polymer on chitosan modified poly (dimethylsiloxane) microchip for separation of biomolecules
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DOI：
10.1016/j.chroma.2007.02.030
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发表时间：
2007-04-13
影响因子：
4.1
通讯作者：
Chen, Hong-Yuan
中科院分区：
文献类型：
--
作者：
Wang, Ai-Jun;Xu, Jing-Juan;Chen, Hong-Yuan
本文提出了一种简单、绿色的聚二甲基硅氧烷（PDMS）微芯片修饰方法，成功地抑制了生物分子的非特异性吸附。在PDMS微通道中，将O-[（N-琥珀酰亚胺基）琥珀酰基]-O '-甲基-聚乙二醇（PEG）原位接枝到预涂壳聚糖（Chit）表面，制备亲水性表面。在碱性介质中，Chit的多糖主链通过疏水作用和氢键结合被强烈地吸引到PDMS表面上。甲基聚乙二醇（mPEG）可以在mPEG/水界面上产生亲水结构域，从而以这种方式产生刷状涂层，并显示出对生物分子非特异性吸附的良好抵抗。该方法可以大大提高生物分子的分离效率和重现性。在铜电极上，氨基酸和蛋白质可以被有效地分离和成功地检测到的涂层芯片耦合端通道安培检测。此外，它为在微流控装置上制备生物相容性和亲水性表面提供了一种有效的手段，可能在生物分析中具有潜在的用途。(c)2007 Elsevier B. V.保留所有权利。
In this paper, a simple and green modification method is developed for biomolecules analysis on poly(dimethylsiloxane) (PDMS) microchip with successful depression of nonspecific biomolecules adsorption. O-[(N-succinimdyl)succiny]-o'-methyl-polyethylene glycol) was explored to form hydrophilic surface via in-situ grafting onto pre-coated chitosan (Chit) from aqueous solution in the PDMS microchannel. The polysaccharide chains backbone of Chit was strongly attracted onto the surface of PDMS via hydrophobic interaction combined with hydrogen bonding in an alkaline medium. The methyl-polyethylene glycol) (mPEG) could produce hydrophilic domains on the mPEG/aqueous interface, which generated brush-like coating in this way and revealed perfect resistance to nonspecific adsorption of biomolecules. This strategy could greatly improve separation efficiency and reproducibility of biomolecules. Amino acids and proteins could be efficiently separated and successfully detected on the coated microchip coupled with end-channel amperometric detection at a copper electrode. In addition, it offered an effective means for preparing biocompatible and hydrophilic surface on microfluidic devices, which may have potential use in the biological analysis. (c) 2007 Elsevier B.V. All rights reserved.