Microarray Expression Profile Analysis of Long Non-Coding RNAs in Umbilical Cord Plasma Reveals their Potential Role in Gestational Diabetes-Induced Macrosomia
Microarray Expression Profile Analysis of Long Non-Coding RNAs in Umbilical Cord Plasma Reveals their Potential Role in Gestational Diabetes-Induced Macrosomia
复制标题
DOI：
10.1159/000430119
复制
发表时间：
2015-05-01
影响因子：
--
通讯作者：
Shen, Rong
中科院分区：
文献类型：
--
作者：
Shi, Zhonghua;Zhao, Chun;Shen, Rong
背景：巨大儿及其相关并发症是妊娠期糖尿病(GDM)婴儿最常见、最严重的并发症。关于长非编码RNA(IncRNAs)与巨大儿之间的相关性的报道很少；因此，我们研究了GDM巨大儿的脐带IncRNA谱。方法：将30对GDM巨大儿和正常对照组随机分为3个亚组，每个亚组的脐带静脉血混合后与含有33000个IncRNA基因的探针芯片杂交。用实时定量聚合酶链式反应(QPCR)验证筛选出的差异表达的IncRNAs。进行基因本体(GO)、通径分析和网络分析。结果：芯片检测到8814个IncRNAs在脐带血中表达，其中GDM组349个表达显著上调，892个表达显著下调(折叠式变化=2.0)。下调的转录本针对的GO丰度最高的是生物调控。途径分析表明，有9条途径对应于下调的转录本。结论：GDM巨大儿脐血中某些IncRNAs的异常表达可能在GDM巨大儿的发生发展中起部分或关键作用。这项研究为巨大儿的治疗提供了潜在的靶点，并为巨大儿生物学提供了新的见解。版权所有(C)2015年S.Karger AG，巴塞尔
Background: Fetal macrosomia and its associated complications are the most frequent and serious morbidities for infants associated with gestational diabetes mellitus (GDM). The associations between long non-coding RNAs (IncRNAs) and macrosomia have been rarely reported; therefore, we investigated the umbilical cord IncRNA profiles in GDM macrosomia. Method: Thirty pairs of GDM macrosomia and normal controls were divided into three subgroups randomly, and the umbilical cord vein blood from each subgroup was mixed, and hybridized to a microarray containing probes representing 33,000 IncRNA genes. Quantitative real-time polymerase chain reaction (qPCR) was used to validate selected differentially expressed IncRNAs. The gene ontology (GO), pathway and network analysis were performed. Result: The microarray identified 8814 IncRNAs that were expressed in the umbilical cord blood, of which 349 were significantly upregulated and 892 were significantly downregulated (fold-change >= 2.0) in GDM group. The highest enriched GOs targeted by downregulated transcripts were biological regulation. Pathway analysis indicated that nine pathways corresponded to downregulated transcripts. Conclusions: Certain IncRNAs that were aberrantly expressed in the umbilical cord blood from GDM macrosomia might play a partial or key role in GDM macrosomia development. This study provided potential targets for treatment of macrosomia and novel insights into macrosomia biology. Copyright (C) 2015 S. Karger AG, Basel