AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance inArtemisia annua
AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance inArtemisia annua
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AaABCG40 提高青蒿素含量并调节青蒿的耐旱性
DOI：
10.3389/fpls.2020.00950
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发表时间：
2020-06-26
影响因子：
5.6
通讯作者：
Tang, Kexuan
中科院分区：
文献类型：
--
作者：
Fu, Xueqing;Liu, Hang;Tang, Kexuan
植物激素脱落酸（阿坝）调节植物的生长、发育和对非生物胁迫的响应，包括衰老、种子萌发、冷胁迫和干旱。多种研究表明，外源阿坝能提高青蒿素含量。annua。一些与阿坝信号相关的转录因子被鉴定通过激活青蒿素合成酶基因来增加青蒿素的积累。然而，在A. annua。在此，我们鉴定了一个多效性耐药（PDR）转运体基因AaPDR 4/AaABCG 40。AaABCG 40主要在根、叶、芽和毛状体中表达。在proAaABCG 40：GUStransgenicA中，GUS活性主要在根和幼叶的维管组织中观察到。一年生植物当AaABCG 40转入酵母AD 12345678中时，表达AaABCG 40的酵母比对照积累更多的阿坝。AaABCG 40过表达植株青蒿素含量高，耐旱性强。此外，OE-AaABCG 40植株中CYP 71 AV 1的表达对外源阿坝的敏感性高于野生型和iAaABCG 40植株。根据这些结果，他们强烈地暗示AaABCG 40参与了阿坝在A. annua。
The phytohormone Abscisic acid (ABA) regulates plant growth, development, and responses to abiotic stresses, including senescence, seed germination, cold stress and drought. Several kinds of researches indicate that exogenous ABA can enhance artemisinin content inA. annua. Some transcription factors related to ABA signaling are identified to increase artemisinin accumulation through activating the artemisinin synthase genes. However, no prior study on ABA transporter has been performed inA. annua. Here, we identified a pleiotropic drug resistance (PDR) transporter geneAaPDR4/AaABCG40fromA. annua.AaABCG40was expressed mainly in roots, leaves, buds, and trichomes. GUS activity is primarily observed in roots and the vascular tissues of young leaves inproAaABCG40:GUStransgenicA. annuaplants. WhenAaABCG40was transferred into yeast AD12345678, yeasts expressingAaABCG40accumulated more ABA than the control. TheAaABCG40overexpressing plants showed higher artemisinin content and stronger drought tolerance. Besides, the expression ofCYP71AV1in OE-AaABCG40plants showed more sensitivity to exogenous ABA than that in both wild-type and iAaABCG40plants. According to these results, they strongly suggest that AaABCG40 is involved in ABA transport inA. annua.