A sensitive chemiluminescent immunoassay to detect Chromotrope FB (Chr FB) in foods
A sensitive chemiluminescent immunoassay to detect Chromotrope FB (Chr FB) in foods
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一种灵敏的化学发光免疫分析法检测食品中的变色剂 FB (Chr FB)
DOI：
10.1016/j.talanta.2016.09.063
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发表时间：
2017
期刊：
影响因子：
6.1
通讯作者：
Xi Rimo
中科院分区：
文献类型：
--
作者：
Xu Kun;Long Hao;Xing Rongge;Yin Yongmei;Eremin Sergei A;Meng Meng;Xi Rimo
变色酸FB（Chr FB）是一种允许用于食品和药品的合成偶氮染料。在中国，Chr FB的每日允许摄入量（ADI）为0 ~ 0.5 mg/kg。本研究合成了一种带有氨基的Chr FB半抗原，制备其人工免疫原。应用新西兰兔抗Chr FB多克隆抗体建立了间接竞争荧光免疫分析法（icCLIA），用于食品中Chr FB的检测。采用辣根过氧化物酶（HRP）-鲁米诺-H_2O_2体系，对碘苯酚为增敏剂，产生化学发光信号。该方法对Chr FB表现出良好的特异性，可以检测低至0.02 ng mL− 1的缓冲液中的Chr FB，0.07 ng g− 1的酸奶糖果，0.07 ng g− 1的维生素饮料和0.13 ng g− 1的面包。与高效液相色谱法相比，该方法的灵敏度提高了两个数量级。该方法的准确度和精密度可接受，与HPLC法相当。该方法可用于上述食品中Chr FB的快速检测。
Chromotrope FB (Chr FB) is a synthetic azo dye permitted for use in foods and medicines. An acceptable daily intake (ADI) of Chr FB was 0–0.5 mg/kg in China. In this study, we synthesized a Chr FB hapten with an amino group to prepare its artificial immunogen. Polyclonal antibodies obtained from New Zealand rabbits were applied to develop an indirect competitive chemiluminescent immunoassay (icCLIA) to detect Chr FB in foods. A horseradish peroxidase (HRP)-luminol-H2O2system was used to yield CL signal with p-iodophenol as an enhancement reagent. The method showed good specificity towards Chr FB and could detect as low as 0.02 ng mL−1Chr FB in buffer, 0.07 ng g−1in yoghurt candy, 0.07 ng g−1in vitamin drink and 0.13 ng g−1in bread. Compared with HPLC method, the proposed method is more sensitive by two orders of magnitude. The accuracy and precision of this method are acceptable and comparable with HPLC method. Therefore, the proposed method could be used for rapid screening of Chr FB in the mentioned foodstuffs.