Behavioral and histological outcomes following graded spinal cord contusion injury in the C57Bl/6 mouse
Behavioral and histological outcomes following graded spinal cord contusion injury in the C57Bl/6 mouse
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DOI：
10.1006/exnr.2001.7679
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发表时间：
2001-06-01
影响因子：
5.3
通讯作者：
Jakeman, LB
中科院分区：
文献类型：
--
作者：
Ma, MH;Basso, DM;Jakeman, LB
采用计算机控制的电磁脊髓损伤装置（ESCID）建立了小鼠脊髓挫伤模型。在本研究中，我们已经扩展了这个模型在C57 B1/6小鼠的行为和组织病理学结果评估。三组小鼠在T1椎骨水平接受椎板切除术，然后从1500达因的预定起始负荷进行挫伤。通过脊髓快速移位至0.3、0.5或0.8 mm的峰值距离产生挫伤，整个损伤和牵开过程在23 ms时间内完成。对照组仅接受椎板切除术或完全横断。使用BBB运动评定量表、网格行走和足迹分析检查损伤后9周的功能恢复。运动恢复的不同模式在五组中是明显的。测量震中的备用白色物质、损伤长度和纤维连接蛋白免疫阳性瘢痕组织的横截面积在损伤组之间也有显著差异。损伤的严重程度与撞击时记录的生物力学测量以及行为和组织学参数相对应。结果表明，分级挫伤可以在小鼠中使用ESCID可靠地产生。这些数据提供了一个彻底的和定量的分析，挫伤损伤的长期行为和组织学结果的措施，在这个应变和物种的影响。(C)北京：科学出版社.
A computer-controlled electromagnetic spinal cord injury device (ESCID) has been adapted to develop a mouse model of spinal cord contusion injury. In the present study, we have extended this model in C57Bl/6 mice with behavioral and histopathological outcome assessment. Three groups of mice received a laminectomy at the T, vertebral level followed by a contusion injury from a predetermined starting load of 1500 dynes, Contusion was produced by rapid displacement of the spinal cord to a peak distance of 0.3, 0.5, or 0.8 mm, with the entire injury and retraction procedure completed over a 23-ms epoch. Control groups received laminectomy alone or complete transection. Functional recovery was examined for 9 weeks after injury using the BBB locomotor rating scale, grid walking, and footprint analysis. Distinct patterns of locomotor recovery were evident across the five groups. Measurements of spared white matter at the epicenter, lesion length, and cross-sectional area of fibronectin-immunopositive scar tissue were also significantly different between injury groups. The severity of injury corresponded with the biomechanical measures recorded at the time of impact as well as with behavioral and histological parameters. The results demonstrate that graded contusion injuries can be produced reliably in mice using the ESCID. The data provide a thorough and quantitative analysis of the effects of contusion injury on long-term behavioral and histological outcome measures in this strain and species. (C) 2001 Academic Press.