Seven new microcystin variants discovered from a native Microcystis aeruginosa strain - unambiguous assignment of product ions by tandem mass spectrometry
Seven new microcystin variants discovered from a native Microcystis aeruginosa strain - unambiguous assignment of product ions by tandem mass spectrometry
复制标题
DOI：
10.1002/rcm.7098
复制
发表时间：
2015-01-30
影响因子：
2
通讯作者：
Volmer, Dietrich A.
中科院分区：
文献类型：
--
作者：
Qi, Yulin;Rosso, Lorena;Volmer, Dietrich A.
基本原理：采用高分辨质谱技术对一株产[D-Leu（1）]MC-LR的铜绿微囊藻进行了研究。详细的分析揭示了新的微囊藻毒素（MC）变种产生的菌株，其中7个是以前未报告的变种。这项工作显示了质量准确度的重要性，为识别未知的MC。铜绿假单胞菌菌株分离自从阿根廷收集的水华样品，并适应实验室条件。采用紫外/维斯检测引导的高效液相色谱分离菌株中的MC变异体，并采用串联质谱（MS/MS）确定其结构。MS/MS分析揭示了10个MC的变种产生的菌株，其中7个从来没有被reportedbefore.CONCLUSIONS：这项工作显示了同量异位素和异构体的干扰，在未知的MC的研究，因此，高质量的准确性是很重要的，以避免错误的分配。此外，这里提供的峰列表（30-50个片段明确地分配给10个MC）可以用作从环境样品中发现MC的参考。版权所有（c）2014约翰威利父子有限公司
RATIONALE: High-resolution mass spectrometry was applied to the study of a Microcystis aeruginosa strain previously reported as a [D-Leu(1)]MC-LR producer. Detailed analysis revealed new microcystin (MC) variants produced from the strain, and seven of these were previously unreported variants. This work shows the importance of mass accuracy for the identification of unknown MCs.METHODS: The M. aeruginosa strain was isolated from a bloom sample collected from Argentina and acclimated to lab conditions. The MC variants in the strain were separated by UV/Vis detection-guided high-performance liquid chromatography, and their structures were unambiguous determined by tandem mass spectrometry (MS/MS).RESULTS: A simple strategy was developed for quickly locating the low-abundance MC precursors from complex samples. MS/MS anlysis revealed ten MC variants produced from the strain, of which seven have never been reported before.CONCLUSIONS: This work shows the interference of isobarics and isomers in the study of unknown MCs, and, therefore, high mass accuracy is important to avoid false assignments. Moreover, the peak list provided here (30-50 fragments unambiguously assigned for ten MCs) can be used as a reference for the discovery of MCs from environmental samples. Copyright (c) 2014 John Wiley & Sons, Ltd.