Slm9, a novel nuclear protein involved in mitotic control in fission yeast.
Slm9, a novel nuclear protein involved in mitotic control in fission yeast.
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Slm9，一种参与裂殖酵母有丝分裂控制的新型核蛋白。
DOI：
10.1093/genetics/155.2.623
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Russell,P
中科院分区：
文献类型：
--
作者：
Kanoh,J;Russell,P
在裂殖酵母裂殖酵母中，如同在其他真核细胞中一样，Cdc 2/cyclin B复合物是有丝分裂的关键调节因子。也许Cdc 2最重要的调节是由Wee 1和Mik 1催化的酪氨酸-15的抑制性磷酸化。Cdc 25和Pyp 3磷酸酶使酪氨酸-15去磷酸化并激活Cdc 2。为了分离新型Cdc 2激酶激活剂，我们在acdc 25 - 22背景下在允许温度（25°）下筛选合成致死突变体。其中一个基因slm 9编码一种新的807个氨基酸的蛋白质。slm 9与芽殖酵母中的组蛋白基因调节子Hir 2最相似。Slm 9蛋白水平是恒定的，并且Slm 9在整个细胞周期中定位于细胞核。细胞伸长和DNA含量分析表明，lm 9破坏剂在G2-M转换时延迟。Wee 1的失活完全抑制了lm 9突变引起的细胞伸长表型。lm 9突变体在氮饥饿后从G1期阻滞中恢复的能力有缺陷。该lm 9突变体也是紫外线敏感的，表现出缺陷，在恢复从细胞周期停滞后，紫外线照射。
In the fission yeastSchizosaccharomyces pombe, as in other eukaryotic cells, Cdc2/cyclin B complex is the key regulator of mitosis. Perhaps the most important regulation of Cdc2 is the inhibitory phosphorylation of tyrosine-15 that is catalyzed by Wee1 and Mik1. Cdc25 and Pyp3 phosphatases dephosphorylate tyrosine-15 and activate Cdc2. To isolate novel activators of Cdc2 kinase, we screened synthetic lethal mutants in acdc25-22background at the permissive temperature (25°). One of the genes,slm9, encodes a novel protein of 807 amino acids. Slm9 is most similar to Hir2, the histone gene regulator in budding yeast. Slm9 protein level is constant and Slm9 is localized to the nucleus throughout the cell cycle. Theslm9disruptant is delayed at the G2-M transition as indicated by cell elongation and analysis of DNA content. Inactivation of Wee1 fully suppressed the cell elongation phenotype caused by theslm9mutation. Theslm9mutant is defective in recovery from G1arrest after nitrogen starvation. Theslm9mutant is also UV sensitive, showing a defect in recovery from the cell cycle arrest after UV irradiation.