Fluorescent chemodosimeter for Cys/Hcy with a large absorption shift and imaging in living cells
Fluorescent chemodosimeter for Cys/Hcy with a large absorption shift and imaging in living cells
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具有大吸收位移和活细胞成像的 Cys/Hcy 荧光化学剂量计
DOI：
10.1039/c0ob00957a
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发表时间：
2011-01-01
影响因子：
3.2
通讯作者：
Peng, Xiaojun
中科院分区：
文献类型：
--
作者：
Hu, Mingming;Fan, Jiangli;Peng, Xiaojun
设计了一种具有高效分子内电荷转移的新型分子T1，用于半胱氨酸（Cys）和同型半胱氨酸（Hcy）的荧光化学剂量计。加入半胱氨酸/同型半胱氨酸后，T1的荧光强度大大增强，吸收峰位移较大（70 nm），可用于活细胞中半胱氨酸/同型半胱氨酸的生物成像和人血浆中半胱氨酸/同型半胱氨酸的检测。通过H-1 NMR、质谱分析和高斯计算证实了检测机理。
A novel molecule T1 with efficient intramolecular charge transfer was designed as a fluorescent chemodosimeter for cysteine (Cys) and homocysteine (Hcy). Upon addition of Cys/Hcy, T1 exhibited greatly enhanced fluorescence intensity as well as a large absorption peak shift (70 nm), and can be used for bioimaging of Cys/Hcy in living cells and detection in human plasma by visual color change. The detection mechanism was proved by H-1 NMR, mass spectrometry analysis and Gaussian calculations.