Molecular Imprinting of Luminescent Vesicles
Molecular Imprinting of Luminescent Vesicles
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DOI：
10.1021/ja4001568
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发表时间：
2013-02-27
影响因子：
15
通讯作者：
Koenig, Burkhard
中科院分区：
文献类型：
--
作者：
Banerjee, Supratim;Koenig, Burkhard
将分子印迹技术应用于功能化单层流体囊泡表面，可以制备特异性和高亲和力的发光化学传感器。我们有光聚合的丁二炔含有囊泡中的小肽作为模板产生印迹聚丁二炔（PDA）补丁的膜。它们作为多价受体位点，对模板的再结合亲和力显著增加。所有结合位点都是表面暴露的，并且可用于分析物结合。通过共嵌入流体DOPC膜中的两亲性羧基荧光素的发射强度变化，以高灵敏度发出分析物的存在的信号。PDA印迹与动态功能化囊泡的合并克服了目前分子印迹在化学传感器设计中的一些局限性，并可应用于许多不同的目标分析物。
Applying molecular imprinting techniques to the surface of functionalized unilammelar fluid vesicles allows the preparation of specific and high-affinity luminescent chemosensors. We have photopolymerized diacetylene containing vesicles in the presence of small peptides as templates yielding imprinted polydiacetylene (PDA) patches in the membrane. They serve as multivalent receptor sites with significantly increased rebinding affinity for the template. All binding sites are surface exposed and accessible for analyte binding. The presence of analytes is signaled with high sensitivity by emission intensity changes of amphiphilic carboxyfluorescein, which is coembedded into the fluid DOPC membrane. The merger of PDA imprinting with dynamic functionalized vesicles overcomes some of the current limitations of molecular imprinting in chemosensor design and may be applied to many different target analytes.