Intracellular Presence of Insulin and Its Phosphorylated Receptor in Non-Small Cell Lung Cancer
Intracellular Presence of Insulin and Its Phosphorylated Receptor in Non-Small Cell Lung Cancer
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DOI：
10.1002/jcp.21916
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发表时间：
2009-12-01
影响因子：
5.6
通讯作者：
Paggi, Marco G.
中科院分区：
文献类型：
--
作者：
Mattarocci, Stefano;Abbruzzese, Claudia;Paggi, Marco G.
胰岛素长期以来一直被认为影响正常和转化细胞的生长和分化。为了明确胰岛素在非小细胞肺癌（NSCLC）中的作用，我们现在通过免疫组织化学（IHC）搜索NSCLC样本中胰岛素的存在。在我们研究的112个样品中，发现30个含有胰岛素，其以胞浆内颗粒的形式被检测到。此外，其表达与以下因素显著相关：（a）NSCLC的形态学/组织病理学亚型，在腺癌中更常见;（B）肿瘤分化等级，显示低级别癌的增加;（c）肿瘤大小，主要发生在较小的肿瘤中;（d）磷酸化、活化胰岛素受体的存在;（e）患者中位年龄，存在于相对年轻的个体中。此外，有趣的是，周围的非典型腺瘤增生区和正常肺泡肺泡上皮细胞在一些胰岛素阴性肿瘤中呈胰岛素阳性。此外，在免疫反应性胰岛素阳性的一些样本中，胰岛素转录物的PCR检测结果为阴性，表明我们的NSCLC队列中细胞内胰岛素可能为外源性来源。总之，我们的数据表明，细胞内胰岛素活性是重要的低级别人肺腺癌的进展。J.细胞。221：766-770，2009。(C)2009威利-利斯公司
Insulin has been known for a longtime to influence the growth and differentiation of normal and transformed cells. In order to delineate the role of insulin specifically in non-small cell lung cancer (NSCLC), we have now searched by immunohistochemistry (IHC) for the presence of insulin in NSCLC samples. Among the 112 samples we studied, 30 were found to contain insulin, which was detected in the form of intracytoplasmic granula. Moreover, its expression significantly correlated with (a) the morphological/histopathological subtype of NSCLC, being more frequent in adenocarcinomas; (b) the grade of tumor differentiation, displaying an increase in low-grade carcinomas; (c) tumor size, occurring predominantly in smaller tumors; (d) the presence of phosphorylated, activated insulin receptor; (e) the median patient age, being present in relatively younger individuals. Furthermore and interestingly, surrounding atypical adenomatous hyperplastic areas and normal alveolar pneumocytes scored insulin-positive in some of the insulin-negative tumors. In addition, PCR exploration for insulin transcripts in some samples positive for immunoreactive insulin was negative, indicating a possibly exogenous origin for the intracellular insulin in our NSCLC cohort. Taken together, our data suggest that an intracellular insulin activity is important for the progression of low-grade human lung adenocarcinomas. J. Cell. Physiol. 221: 766-770, 2009. (C) 2009 Wiley-Liss, Inc.