Research on the neuro-protective compounds in Terminalia chebula retz extracts in-vivo by UPLC–QTOF-MS
Research on the neuro-protective compounds in Terminalia chebula retz extracts in-vivo by UPLC–QTOF-MS
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DOI：
10.1556/1326.2017.00147
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发表时间：
2017-08
影响因子：
1.9
通讯作者：
Yuanyuan Zhang;Xiaohui Liu;Gao Shuting;K. Qian;Qingshan Liu;Ying Xiaoying
中科院分区：
文献类型：
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作者：
Yuanyuan Zhang;Xiaohui Liu;Gao Shuting;K. Qian;Qingshan Liu;Ying Xiaoying
采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-QTOF-MS）对诃子提取物灌胃给药后大鼠血浆和脑组织中的吸收成分进行分析。血浆中鉴定出9种组分（没食子酸、没食子酸甲酯、阿魏酸、没食子酸乙酯、短叶苏木素羧酸、鞣花酸、没食子黄素、arjugenin和arjunic acid）和4种代谢产物，大鼠脑中鉴定出5种组分（没食子酸乙酯、短叶苏木素羧酸、鞣花酸、arjugenin和arjunic acid），基于它们的碎裂行为。血浆中存在的成分与T.诃子Retz，并在大脑中吸收的成分与其神经保护作用。这种方法使我们能够快速确定T.并建立其质量控制方法。该分析方法表明，G…
We have developed a strategy to analyze the components absorbed in the plasma and brain tissue of rats after intragastric administration of Terminalia chebula Retz extracts by ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC–QTOF-MS). Nine components (gallic acid, methyl gallate, ferulic acid, ethyl gallate, brevifolin carboxylic acid, ellagic acid, galloflavin, arjugenin, and arjunic acid) and four metabolites were identified in plasma, and five components (ethyl gallate, brevifolin carboxylic acid, ellagic acid, arjugenin, and arjunic acid) were identified in the rat brain based on their fragmentation behaviors. The components present in the plasma were associated with the antioxidant activity of T. chebula Retz, and the components absorbed in the brain were associated with its neuro-protective effects. This approach allowed us to rapidly determine the active components of T. chebula Retz and develop a method for its quality control. This analysis method showed g...