cDNA cloning, tissue expression, and chromosome mapping of human homolog of SOX18
cDNA cloning, tissue expression, and chromosome mapping of human homolog of SOX18
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DOI：
10.1007/s100380050210
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发表时间：
2000-01-01
影响因子：
3.5
通讯作者：
Muramatsu, M
中科院分区：
文献类型：
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作者：
Azuma, T;Seki, N;Muramatsu, M
SRY（性别决定区Y）基因编码一种转录因子，其特征在于被称为HMG（高迁移率族）结构域的DNA结合基序。SOX（Sry-box）基因包含与HMG-盒区域同源的大家族。我们分离了一个具有开放阅读框的cDNA克隆，其编码384个氨基酸的推定蛋白，与小鼠Sox 18蛋白具有83%的同一性。北方印迹分析显示，1.9 kb的人SOX 18信使RNA主要在心脏中表达，尽管在脑、肝、睾丸和白细胞中观察到弱信号。通过聚合酶链反应（PCR）为基础的分析与人类/啮齿动物单染色体杂交细胞面板和辐射杂交面板，该基因被定位到染色体20 q13.33区域。
The SRY (sex-determining region Y) gene encodes a transcription factor characterized by a DNA-binding motif termed the HMG (high mobility group) domain. The SOX (Sry-box) genes comprise a large family related by homology to the HMG-box region. We isolated a cDNA clone with an open reading frame encoding a putative protein of 384 amino acids, which shared 83% identity to the mouse Sox18 protein. Northern blot analysis revealed that a 1.9-kb band of human SOX18 messenger RNAs was predominantly expressed in heart, although weak signals were seen in brain, liver, testis, and leukocyte. By poly merase chain reaction (PCR)-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 20q13.33 region.