OVERLAPPING ELEMENTS IN THE GUANYLATE-BINDING PROTEIN GENE PROMOTER MEDIATE TRANSCRIPTIONAL INDUCTION BY ALPHA AND GAMMA INTERFERONS
OVERLAPPING ELEMENTS IN THE GUANYLATE-BINDING PROTEIN GENE PROMOTER MEDIATE TRANSCRIPTIONAL INDUCTION BY ALPHA AND GAMMA INTERFERONS
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DOI：
10.1128/mcb.11.1.182
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发表时间：
1991-01-01
影响因子：
5.3
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
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作者：
LEW, DJ;DECKER, T;DARNELL, JE
编码67 kda细胞质鸟苷酸结合蛋白（GBP）的基因在暴露于I型（α干扰素[inf - α]或inf - β）或II型（ifn - γ）干扰素的细胞中被转录诱导。克隆GBP基因的启动子，发现含有ifn - α刺激反应元件，介导GBP基因对ifn - α的反应。在重组质粒转染实验的基础上，划分了两种不同的元素。要获得GBP基因对ifn - γ的最大反应，这两个元素都是必需的：ifn - α刺激的反应元件和称为ifn - γ激活位点的重叠元件。研究了作用于每个元件的不同蛋白质，并讨论了它们可能参与ifn - γ诱导的转录调节。
The gene encoding a 67-kDa cytoplasmic guanylate-binding protein (GBP) is transcriptionally induced in cells exposed to interferon of either type I (alpha interferon [INF-alpha] or INF-beta) or type II (IFN-gamma). The promoter of the GBP gene was cloned and found to contain an IFN-alpha-stimulated response element, which mediated the response of the GBP gene to IFN-alpha. On the basis of transfection experiments with recombinant plasmids, two different elements were delineated. Both were required to obtain the maximal response of the GBP gene to IFN-gamma: the IFN-alpha-stimulated response element and an overlapping element termed the IFN-gamma activation site. Different proteins that act on each element were investigated, and their possible involvement in IFN-gamma-induced transcriptional regulation is discussed.