Morning urinary assessment of nocturnal melatonin secretion in older women
Morning urinary assessment of nocturnal melatonin secretion in older women
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DOI：
10.1034/j.1600-079x.2000.280106.x
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发表时间：
2000-01-01
影响因子：
10.3
通讯作者：
Kheifets, L
中科院分区：
文献类型：
--
作者：
Cook, MR;Graham, C;Kheifets, L
我们评估了在流行病学研究中使用晨尿样本的可行性，目的是澄清夜间褪黑激素水平与乳腺癌风险之间的关系。最初，对29名女性（40-70岁）进行了一项基于实验室的研究，以检查每小时夜间血液样本中血浆褪黑素水平与晨尿样本中褪黑素及其主要酶代谢产物6-羟褪黑素硫酸酯（6-OHMS）之间的相关性。在一项伴随的实地研究中，收集了203名健康妇女的晨尿样本，以评估实验室测量与实地测量的异同。综上所述，我们的研究结果表明：1)第一次晨空尿中褪黑素和肌酐校正的6-OHMS水平与夜间血浆褪黑素总输出量（P < 0.001）以及夜间褪黑素峰值值（P < 0.001）密切相关；2) 6-OHMS在实验室和现场的范围相似；3)绝经状态和激素替代治疗均未改变晨空尿6-OHMS值。将晨尿样本纳入癌症流行病学研究，可以对褪黑素在人类健康和疾病中所起的作用进行成本效益高的广泛测试。
We evaluated the feasibility of using morning urine samples in epidemiological studies aimed at clarifying the relationship between nocturnal melatonin levels and breast cancer risk. Initially, a laboratory-based study of 29 women (40-70 yr old) was performed to examine the correlation between plasma melatonin levels in hourly nocturnal blood samples and both melatonin and its major enzymatic metabolite, 6-hydroxymelatonin-sulfate (6-OHMS) in morning urine samples. In a companion field study, morning urine samples were collected from 203 healthy women to assess similarities and differences in laboratory versus field measures. Taken together, our results indicate: 1) levels of melatonin and of creatinine-corrected 6-OHMS in the first morning void urine are strongly correlated with total nocturnal plasma melatonin output (P < 0.001) and also with peak nocturnal melatonin values (P < 0.001); 2) similar ranges for 6-OHMS were found in the laboratory and the field; and 3) neither menopausal status nor hormonal replacement therapy altered 6-OHMS values in morning void urine. The inclusion of morning urine samples in epidemiological studies of cancer could allow cost-effective, widespread testing of the role played by melatonin in human health and disease.