Adherence of human immunodeficiency virus-infected lymphocytes to fetal placental cells: a model of maternal --> fetal transmission.
Adherence of human immunodeficiency virus-infected lymphocytes to fetal placental cells: a model of maternal --> fetal transmission.
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人类免疫缺陷病毒感染的淋巴细胞粘附到胎儿胎盘细胞：母体->胎儿传播模型。
DOI：
10.1073/pnas.92.4.978
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发表时间：
1995
影响因子：
11.1
通讯作者：
Blakemore,K
中科院分区：
文献类型：
--
作者：
Schwartz,DH;Sharma,UK;Perlman,EJ;Blakemore,K
子宫内人类免疫缺陷病毒（HIV）感染的确切时间和机制尚不清楚，但经胎盘传播是可能的。来自HIV+妊娠的足月胎盘仅含有罕见的HIV感染细胞，其来源和表型仍存在争议，并且在足月胎盘中存在HIV与传播给后代之间没有发现相关性。滋养层细胞感染性的报道是不可重复的，并且没有解决胎盘间质（与胎儿循环直接接触的细胞）感染的问题。我们报告说，原代培养的胎儿胎盘绒毛膜绒毛基质细胞，而不是在体外感染，支持致命照射的艾滋病毒感染的外周血单核细胞（PBMC）的形式，允许救援的艾滋病毒激活的PBMC周后。受感染的PBMC粘附并通过LFA-1和CD 4独立的机制与胎盘细胞密切相关，但可以通过抗体或可溶性CD 4与细胞表面表达的HIV包膜结合来阻断。几种滋养层细胞、成纤维细胞或上皮细胞系不具有维持受感染的辐照细胞的能力。该模型具有与子宫内传播相容的几个特征，并允许测试作为干预措施的各种试剂，以阻断母体->胎儿传播。胎盘基质细胞似乎抑制HIV感染的辐射淋巴细胞的凋亡。
The precise timing and mechanism of in utero human immunodeficiency virus (HIV) infection are unknown, but transplacental transmission is likely. Term placentas from HIV+ pregnancies contain only rare HIV-infected cells whose origins and phenotypes remain controversial, and no correlation has been found between the presence of HIV in term placentas and transmission to offspring. Reports of trophoblast infectibility have not been reproducible and do not address the question of infection in the placental stroma, the cells in direct contact with fetal circulation. We report that primary cultures of fetal placental chorionic villus stromal cells, while not infectable in vitro, do support lethally irradiated HIV-infected peripheral blood mononuclear cells (PBMCs) in a form that permits rescue of HIV by activated PBMCs weeks later. Infected PBMCs adhere and become intimately associated with placental cells by a mechanism that is LFA-1 and CD4 independent but can be blocked by antibodies or soluble CD4 binding to cell surface-expressed HIV envelope. The ability to sustain infected irradiated cells was not shared by several trophoblast, fibroblast, or epithelial cell lines. This model has several features that are compatible with in utero transmission and allow testing of various agents proposed as interventions to block maternal-->fetal transmission. Placental stromal cells appear to inhibit apoptosis of HIV-infected, irradiated lymphocytes.