Neuronal activation in the hypothalamus and brainstem during feeding in rats
Neuronal activation in the hypothalamus and brainstem during feeding in rats
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DOI：
10.1016/j.cmet.2006.08.003
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发表时间：
2006-10-01
期刊：
影响因子：
29
通讯作者：
Leng, Gareth
中科院分区：
文献类型：
--
作者：
Johnstone, Louise E.;Fong, Tung M.;Leng, Gareth
我们训练大鼠的计划喂养，其中食物是每天只有2小时。10天后，在相对于食物可用性的规定时间对大鼠实施安乐死，并分析其大脑以映射神经元群体中的Fos表达，以测试一些群体被饥饿激活而另一些群体被饱足信号激活的假设。Fos的表达伴随着进食在几个下丘脑和脑干核团。食物摄入是关键的Fos表达的去甲肾上腺素能和非去甲肾上腺素能细胞在孤束核和最后区和视上核，以及在黑皮质素细胞的弓状核。然而，对食物的预期单独激活了弓状核和下丘脑外侧和腹内侧的其他神经元，包括食欲素神经元。因此，食欲种群在食物呈现开始时被强烈而迅速地激活，随后在摄食食物时迅速激活食欲种群。
We trained rats to a regime of scheduled feeding, in which food was available for only 2 hr each day. After 10 days, rats were euthanized at defined times relative to food availability, and their brains were analyzed to map Fos expression in neuronal populations to test the hypothesis that some populations are activated by hunger whereas others are activated by satiety signals. Fos expression accompanied feeding in several hypothalamic and brainstem nuclei. Food ingestion was critical for Fos expression in noradrenergic and non-noradrenergic cells in the nucleus tractus solitarii and area postrema and in the supraoptic nucleus, as well as in melanocortin-containing cells of the arcuate nucleus. However, anticipation of food alone activated other neurons in the arcuate nucleus and in the lateral and ventromedial hypothalamus, including orexin neurons. Thus orexigenic populations are strongly and rapidly activated at the onset of food presentation, followed rapidly by activity in anorexigenic populations when food is ingested.