X-ray diffraction from magnetically oriented solutions of macromolecular assemblies.
X-ray diffraction from magnetically oriented solutions of macromolecular assemblies.
复制标题
大分子组装体磁定向溶液的 X 射线衍射。
DOI：
10.1126/science.3945822
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Makowski,L
中科院分区：
文献类型：
--
作者：
Glucksman,MJ;Hay,RD;Makowski,L
开发了一个简单的系统，用于从大分子组装体的磁定向溶液中获取 X 射线衍射图案。设计了一种小型永磁体，可在 1 立方毫米的体积中产生 16 千高斯的磁场，并且可安装在大多数 X 射线相机上。许多亚细胞结构具有足够的抗磁各向异性，当放置在磁体的两极之间时，它们在稀溶液中表现出取向。与各向同性溶液的小角度散射相比，该磁体中定向溶液的衍射可以提供更多的结构信息。在有利的情况下，例如丝状噬菌体的稀溶液，可以产生定向纤维衍射图案，通过该图案可以测量沿层线的强度至 7 埃的分辨率。在其他大分子组装体的溶液中观察到的磁致双折射表明该技术可能对亚细胞结构具有广泛的适用性。
A simple system was developed for obtaining x-ray diffraction patterns from magnetically oriented solutions of macromolecular assemblies. A small permanent magnet was designed that produces a magnetic field of 16 kilogauss in a volume of 1 cubic millimeter and is mountable on most x-ray cameras. Many subcellular structures have sufficient diamagnetic anisotropy that they exhibit orientation in dilute solution when placed between the poles of the magnet. Diffraction from solutions oriented in this magnet can provide substantially more structural information than small-angle scattering from isotropic solutions. In favorable cases, such as dilute solutions of filamentous bacteriophages, it is possible to produce oriented fiber diffraction patterns from which intensities along layer lines can be measured to 7-angstrom resolution. The magnetically induced birefringence observed in solutions of other macromolecular assemblies suggests that this technique may have broad applicability to subcellular structures.