Size exclusion and diffusion of fluoresceinated probes within collagen fibrils
Size exclusion and diffusion of fluoresceinated probes within collagen fibrils
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DOI：
10.1103/physreve.67.021909
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发表时间：
2003-02-01
影响因子：
2.4
通讯作者：
Fygenson, DK
中科院分区：
文献类型：
--
作者：
Ekani-Nkodo, A;Fygenson, DK
荧光探针在单个胶原原纤维中的扩散是通过成像荧光在油中沿原纤维的迁移和监测光漂白后的荧光恢复（FRAP）来研究的。根据探针的大小将其从原纤维中排除。未排除的探针在原纤维中扩散，但由于胶原与探针之间的结合相互作用，FRAP的发生速度比在水中慢6 × 10（-4）倍。测定了荧光素-胶原复合物的解离常数（K-D=1.8+/-0.1 muM）。
The diffusion of fluoresceinated probes inside single collagen fibrils was investigated by imaging the migration of fluorescence along the fibrils in oil and by monitoring fluorescence recovery after photobleaching (FRAP). Probes were excluded from the fibrils according to their size. Probes that were not excluded diffused in the fibrils, but FRAP occurred 6x10(-4) times more slowly than in water due to binding interactions between collagen and the probes. The dissociation constant of the fluorescein-collagen complex was determined (K-D=1.8+/-0.1 muM).